Human ADAM metallopeptidase with thrombospondin type 1 motif, 1 ELISA Kit | ADAMTS1 elisa kit

Human A Disintegrin and Metalloproteinase with Thrombospondin Motifs 1, ADAMTS1 ELISA Kit

Cat. Num. AAA705598

• Gene Names: ADAMTS1; C3-C5; METH1
• Reactivity: Human
• Synonyms: ADAM metallopeptidase with thrombospondin type 1 motif; 1; Human A Disintegrin and Metalloproteinase with Thrombospondin Motifs 1; ADAMTS1 ELISA Kit; Human A Disintegrin and Metalloproteinase with Thrombospondin Motifs 1 (ADAMTS1) ELISA kit; C3-C5; KIAA1346; METH1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; human metalloproteinase with thrombospondin type 1 motifs; ADAMTS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ADAMTS1. No significant cross-reactivity or interference between Human ADAMTS1 and analogues was observed.
• Sequence Length: 967

• Samples: Serum, plasma, tissue homogenates and urine
• Assay Type: Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAMTS1 elisa kit

For Sample For the quantitative determination of human A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) concentrations in serum, plasma, tissue homogenates and urine.

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 50845384
• NCBI GeneID: 9510
• NCBI Accession #: NP_008919.3
• NCBI GenBank Nucleotide #: NM_006988.3
• UniProt Accession #: Q9UHI8; Q9NSJ8; Q9P2K0; Q9UH83; Q9UP80; D3DSD5
• Molecular Weight: 105,358 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 1 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 1
• NCBI Official Symbol: ADAMTS1
• NCBI Official Synonym Symbols: C3-C5; METH1
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 1; METH-1; ADAM-TS1; ADAMTS-1; ADAM-TS 1; human metalloproteinase with thrombospondin type 1 motifs; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 1
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS1
• UniProt Synonym Gene Names: KIAA1346; METH1; ADAM-TS 1; ADAM-TS1; ADAMTS-1
• UniProt Entry Name: ATS1_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.1 PublicationManual assertion based on experiment in:Ref.2

Catalytic activity: Cleaves aggrecan at the 1938-Glu-|-Leu-1939 site, within the chondroitin sulfate attachment domain.

Cofactor: Binds 1 zinc ion per subunit.1 PublicationManual assertion based on experiment in:Ref.8