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Western Blot (WB) (Western blot analysis of extracts from HEPG2 cells and COLO cells, using ACRO (heavy chain, Cleaved-Ile43) antibody.)

anti-Human ACRO Antibody | anti-ACRO antibody

ACRO (heavy chain, Cleaved-Ile43) Antibody

Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ACRO; ACRO (heavy chain; Cleaved-Ile43) Antibody; Acrosin; EC=3.4.21.10; anti-ACRO antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
ACRO (heavy chain, Cleaved-Ile43) antibody detects endogenous levels of fragment of activated ACRO resulting from cleavage adjacent to Ile43.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
421
Applicable Applications for anti-ACRO antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized peptide derived from human ACRO.
All Sites
Human: Ile43
Preparation and Storage
Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts from HEPG2 cells and COLO cells, using ACRO (heavy chain, Cleaved-Ile43) antibody.)

Western Blot (WB) (Western blot analysis of extracts from HEPG2 cells and COLO cells, using ACRO (heavy chain, Cleaved-Ile43) antibody.)
References
Baba T., FEBS Lett. 244:296-300(1989) [PubMed: 2493394]. Keime S., Eur. J. Biochem. 190:195-200(1990) [PubMed: 2114285]. Adham I.M., Hum. Genet. 84:125-128(1990) [PubMed: 2298447]. Keime S., Eur. J. Biochem. 190:195-200(1990) [PubMed: 2114285]. Adham I.M., Hum. Genet. 84:125-128(1990) [PubMed: 2298447].

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
49
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45,847 Da
NCBI Official Full Name
acrosin
NCBI Official Synonym Full Names
acrosin
NCBI Official Symbol
ACR
NCBI Protein Information
acrosin
UniProt Protein Name
Acrosin
Protein Family
UniProt Gene Name
ACR
UniProt Synonym Gene Names
ACRS

NCBI Description

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008]

Uniprot Description

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Research Articles on ACRO

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Product Notes

The ACRO acr (Catalog #AAA856221) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The ACRO (heavy chain, Cleaved-Ile43) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACRO can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:1000. Researchers should empirically determine the suitability of the ACRO acr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACRO, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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