SDS-Page
(METTL3 antibody (mAb) tested by Western blot 40 ug of K562 cytoplasmic extract was run on SDS-PAGE and probed with antibody at a dilution of 1:500. In addition, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.)
Mouse anti-Human METTL3 Monoclonal Antibody | anti-METTL3 antibody
METTL3 antibody (mAb)(Clone 29C8)
Gene Names
METTL3; M6A; IME4; Spo8; MT-A70
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Synonyms
METTL3; Monoclonal Antibody; METTL3 antibody (mAb)(Clone 29C8); Methyltransferase Like 3; MRNA (2-O-Methyladenosine-N(6)-)-Methyltransferase; anti-METTL3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
29C8
Form/Format
This antibody is provided as a hybridoma culture supernatant with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-METTL3 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:1000 dilution
This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody
WB: 1:500-1:1000 dilution
This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody
Immunogen
This antibody was raised against a peptide within the N-terminal region of human METTL3 (N6-adenosine-methyltransferase).
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(METTL3 antibody (mAb) tested by Western blot 40 ug of K562 cytoplasmic extract was run on SDS-PAGE and probed with antibody at a dilution of 1:500. In addition, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.)
SDS-Page
(METTL3 antibody (mAb) tested by Western blot 40 ug of K562 cytoplasmic extract was run on SDS-PAGE and probed with antibody at a dilution of 1:500. In addition, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.)
Related Product Information for anti-METTL3 antibody
Short Description: Our METTL3 antibody (mAb)(Clone 29C8) was raised in a Mouse host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also occurs in other RNA molecules, such as primary miRNA (pri-miRNAs). METTL3 (methyltransferase-like 3, also known as IME4, M6A, MT-A70) forms a stable N6-methyltransferase heterodimer complex with METTL14, which catalyzes the generation of m6A modification on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition. METTL3 promotes translation by interacting with the translation initiation machinery in the cytoplasm. Its overexpression in a number of cancer cells suggests that it may participate to cancer cell proliferation by promoting mRNA translation.
Background: N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also occurs in other RNA molecules, such as primary miRNA (pri-miRNAs). METTL3 (methyltransferase-like 3, also known as IME4, M6A, MT-A70) forms a stable N6-methyltransferase heterodimer complex with METTL14, which catalyzes the generation of m6A modification on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition. METTL3 promotes translation by interacting with the translation initiation machinery in the cytoplasm. Its overexpression in a number of cancer cells suggests that it may participate to cancer cell proliferation by promoting mRNA translation.
Product Categories/Family for anti-METTL3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,479 Da
NCBI Official Full Name
N6-adenosine-methyltransferase 70 kDa subunit
NCBI Official Synonym Full Names
methyltransferase like 3
NCBI Official Symbol
METTL3
NCBI Official Synonym Symbols
M6A; IME4; Spo8; MT-A70
NCBI Protein Information
N6-adenosine-methyltransferase 70 kDa subunit; adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; methyltransferase-like protein 3
UniProt Protein Name
N6-adenosine-methyltransferase 70 kDa subunit
Protein Family
UniProt Gene Name
METTL3
UniProt Synonym Gene Names
MTA70; MT-A70
UniProt Entry Name
MTA70_HUMAN
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Product Notes
The METTL3 mettl3 (Catalog #AAA389057) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The METTL3 antibody (mAb)(Clone 29C8) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's METTL3 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: WB: 1:500-1:1000 dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody. Researchers should empirically determine the suitability of the METTL3 mettl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "METTL3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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