Testing Data
Mouse Methionine-tRNA Synthetase Monoclonal Antibody | anti-MARS antibody
Methionine-tRNA Synthetase (MARS, METRS, MTRNS)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Flow Cytometry: 0.5-2ug/ml
Western Blot: 0.5-2ug/ml
Immunocytochemistry: 2-50ug/ml
Testing Data
Testing Data
Western Blot (WB)
(Detection of MARS by Western blot. Samples: Whole cell lysate from human HEK293 (H, 25ug) , mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells. Predicted molecular weight: 101 kD)
Western Blot (WB)
(Detection of MARS by Western blot. Samples: Whole cell lysate from human HEK293 (H, 25ug) , mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells. Predicted molecular weight: 101 kD)
Immunoprecipitation (IP)
(Immunoprecipitation RIPA lysate of HeLa cells was incubated with MBS603933)
Immunoprecipitation (IP)
(Immunoprecipitation RIPA lysate of HeLa cells was incubated with MBS603933)
Testing Data
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with M3022-50b (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488- conjugated goat anti-mouse IgG.)
Testing Data
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with M3022-50b (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488- conjugated goat anti-mouse IgG.)
Immunostaining (IS)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS603933)
Immunostaining (IS)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS603933)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq]
Uniprot Description
MARS: a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011]
Protein type: Translation; EC 6.1.1.10; Other Amino Acids Metabolism - selenoamino acid; Ligase
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: mitochondrion; membrane; cytoplasm; cytosol
Molecular Function: methionine-tRNA ligase activity; ATP binding; tRNA binding
Biological Process: tRNA aminoacylation for protein translation; methionyl-tRNA aminoacylation; gene expression
Disease: Infantile Liver Failure Syndrome 2; Charcot-marie-tooth Disease, Axonal, Type 2u
Research Articles on MARS
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Product Notes
The MARS mars (Catalog #AAA603933) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Methionine-tRNA Synthetase (MARS, METRS, MTRNS) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Methionine-tRNA Synthetase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Dilution: Flow Cytometry: 0.5-2ug/ml Western Blot: 0.5-2ug/ml Immunocytochemistry: 2-50ug/ml. Researchers should empirically determine the suitability of the MARS mars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Methionine-tRNA Synthetase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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