Western Blot (WB)
(Western blot analysis of MARS Antibody (C-term) in HL-60 cell line lysates (35ug/lane). cMARS (arrow) was detected using the purified Pab.)
Rabbit anti-Human Methionine-tRNA Synthetase, Cytoplasmic Polyclonal Antibody | anti-MARS antibody
Methionine-tRNA Synthetase, Cytoplasmic, CT (Methionyl-tRNA Synthetase, MARS, FLJ35667, Methionine-tRNA Ligase, METRS, MTRNS)
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Western Blot (WB)
(Western blot analysis of MARS Antibody (C-term) in HL-60 cell line lysates (35ug/lane). cMARS (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of MARS Antibody (C-term) in HL-60 cell line lysates (35ug/lane). cMARS (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MARS antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MARS antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011]
Uniprot Description
MARS: a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011]
Protein type: Translation; Other Amino Acids Metabolism - selenoamino acid; EC 6.1.1.10; Ligase
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: mitochondrion; membrane; cytoplasm; cytosol
Molecular Function: methionine-tRNA ligase activity; ATP binding; tRNA binding
Biological Process: tRNA aminoacylation for protein translation; methionyl-tRNA aminoacylation; gene expression
Disease: Infantile Liver Failure Syndrome 2; Charcot-marie-tooth Disease, Axonal, Type 2u
Research Articles on MARS
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Product Notes
The MARS mars (Catalog #AAA630003) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Methionine-tRNA Synthetase, Cytoplasmic, CT (Methionyl-tRNA Synthetase, MARS, FLJ35667, Methionine-tRNA Ligase, METRS, MTRNS) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Methionine-tRNA Synthetase, Cytoplasmic can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the MARS mars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Methionine-tRNA Synthetase, Cytoplasmic, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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