Western Blot (WB)
(Fig.1. Western Blot analysis of various cells using PKC zeta Polyclonal Antibody diluted at 1:2000.)
Rabbit PKC zeta Polyclonal Antibody | anti-PRKCZ antibody
PKC zeta Polyclonal Antibody
IF: 1:200-1:1000
IHC-P: 1:100-1:300
ELISA: 1:5000
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Western Blot (WB)
(Fig.1. Western Blot analysis of various cells using PKC zeta Polyclonal Antibody diluted at 1:2000.)
Western Blot (WB)
(Fig.1. Western Blot analysis of various cells using PKC zeta Polyclonal Antibody diluted at 1:2000.)
Western Blot (WB)
(Fig.2. Western Blot analysis of COS7 cells using PKC zeta Polyclonal Antibody diluted at 1:2000.)
Western Blot (WB)
(Fig.2. Western Blot analysis of COS7 cells using PKC zeta Polyclonal Antibody diluted at 1:2000.)
NCBI and Uniprot Product Information
NCBI Description
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
PKCZ: an AGC kinase of the PKC family. An atypical PKC: its activity is not regulated by Ca2+, PS, DAG or phorbol esters. Constitutively active.
Protein type: EC 2.7.11.13; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; PKC family; Iota subfamily
Chromosomal Location of Human Ortholog: 1p36.33-p36.2
Cellular Component: tight junction; nuclear matrix; apical cortex; leading edge; nuclear envelope; intercellular junction; cytosol; lipid raft; axon hillock; filamentous actin; membrane; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane; microtubule organizing center; cell junction; endosome
Molecular Function: protein serine/threonine kinase activity; protein domain specific binding; protein binding; protein kinase C activity; potassium channel regulator activity; phospholipase binding; insulin receptor substrate binding; metal ion binding; protein kinase binding; ATP binding; protein kinase activity
Biological Process: protein heterooligomerization; membrane hyperpolarization; negative regulation of insulin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; positive regulation of interleukin-4 production; positive regulation of glucose import; positive regulation of T-helper 2 cell differentiation; negative regulation of hydrolase activity; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; vesicle transport along microtubule; inflammatory response; positive regulation of cell-matrix adhesion; platelet activation; negative regulation of peptidyl-tyrosine phosphorylation; phospholipase D activation; cell migration; activation of protein kinase B; microtubule cytoskeleton organization and biogenesis; peptidyl-serine phosphorylation; long-term memory; establishment of cell polarity; actin cytoskeleton reorganization; insulin receptor signaling pathway; positive regulation of insulin receptor signaling pathway; blood coagulation; vascular endothelial growth factor receptor signaling pathway; negative regulation of protein complex assembly; negative regulation of apoptosis
Research Articles on PRKCZ
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Product Notes
The PRKCZ prkcz (Catalog #AAA9700708) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKC zeta Polyclonal Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's PKC zeta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB: 1:500-1:2000 IF: 1:200-1:1000 IHC-P: 1:100-1:300 ELISA: 1:5000. Researchers should empirically determine the suitability of the PRKCZ prkcz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC zeta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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