Western Blot (WB)
(Western blot analysis using MCP1 mouse mAb against truncated MCP-1 recombinant protein.)
Mouse anti-Human MCP-1 Monoclonal Antibody | anti-MCP-1 antibody
MCP-1 Antibody
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-Chromatography
Synonyms
MCP-1; Monoclonal Antibody; MCP-1 Antibody; C-C motif chemokine 2; CCL2; CCL2_HUMAN; Chemokine (C C motif) ligand 2; GDCF 2; GDCF-2; GDCF2; HC11; HSMCR30; JE; MCAF; MCP 1; MCP1; MGC9434; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2 (monocyte chemotactic protein 1; homologous to mouse Sig je); Small inducible cytokine A2; Small inducible cytokine subfamily A (Cys Cys); member 2; Small-inducible cytokine A2; SMC CF; SMC-CF; SMCCF; anti-MCP-1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
MCP-1 antibody detects endogenous levels of total MCP-1
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
99
Applicable Applications for anti-MCP-1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
WB: 1:500-1:2000
Immunogen
Purified recombinant fragment of human MCP-1 expressed in E. Coli
Subcellular Location
Secreted.
Tissue Specificity
Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis using MCP1 mouse mAb against truncated MCP-1 recombinant protein.)
Western Blot (WB)
(Western blot analysis using MCP1 mouse mAb against truncated MCP-1 recombinant protein.)
Related Product Information for anti-MCP-1 antibody
Description: Monocyte chemoattractant protein-1 (MCP-1) is a member of the chemokine-beta family of cytokines. The protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. It may play a inprotant role in the initiation and/or progression of pulmonary hypertension (PH). Blockade of a systemic MCP-1 signal pathway in vivo may prevent PH.
Function: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Subunit Structure: Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans.
Post-translational Modifications: Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
Similarity: Belongs to the intercrine beta (chemokine CC) family.
Function: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Subunit Structure: Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans.
Post-translational Modifications: Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
Similarity: Belongs to the intercrine beta (chemokine CC) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 13 kDa
Predicted: 12 kDa
Predicted: 12 kDa
NCBI Official Full Name
C-C motif chemokine 2
NCBI Official Synonym Full Names
C-C motif chemokine ligand 2
NCBI Official Symbol
CCL2
NCBI Official Synonym Symbols
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
NCBI Protein Information
C-C motif chemokine 2
UniProt Protein Name
C-C motif chemokine 2
Protein Family
UniProt Gene Name
CCL2
UniProt Synonym Gene Names
MCP1; SCYA2; MCAF; MCP-1
NCBI Description
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
Uniprot Description
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Research Articles on MCP-1
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Product Notes
The MCP-1 ccl2 (Catalog #AAA9602811) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MCP-1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MCP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MCP-1 ccl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCP-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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