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Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant MIOX protein (red) or empty vector (blue) stained using MIOX antibody)

Mouse MIOX Monoclonal Antibody | anti-MIOX antibody

MIOX antibody

Gene Names
MIOX; ALDRL6
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
MIOX antibody was purified by affinity chromatography.
Synonyms
MIOX; Monoclonal Antibody; MIOX antibody; Monoclonal MIOX; Anti-MIOX; anti-MIOX antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
4G7
Purity/Purification
MIOX antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
285
Applicable Applications for anti-MIOX antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
IHC: 1:50
WB: 1:2000
Biological Significance
MIOX is an oxidoreductase localised to the kidneys but its function is currently unknown.
Cross-Reactivity
Human
Immunogen
MIOX antibody was raised in mouse using a full length recombinant protein of human MIOX (NP_060054) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant MIOX protein (red) or empty vector (blue) stained using MIOX antibody)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant MIOX protein (red) or empty vector (blue) stained using MIOX antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MIOX protein (Right) or empty vector (Left) detected with MIOX antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MIOX protein (Right) or empty vector (Left) detected with MIOX antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of MIOX protein in paraffin embedded Adenocarcinoma of Human colon tissue using MIOX antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of MIOX protein in paraffin embedded Adenocarcinoma of Human colon tissue using MIOX antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant MIOX protein using MIOX antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant MIOX protein using MIOX antibody)
Related Product Information for anti-MIOX antibody
Mouse monoclonal MIOX antibody
Product Categories/Family for anti-MIOX antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,516 Da
NCBI Official Full Name
inositol oxygenase
NCBI Official Synonym Full Names
myo-inositol oxygenase
NCBI Official Symbol
MIOX
NCBI Official Synonym Symbols
ALDRL6
NCBI Protein Information
inositol oxygenase
UniProt Protein Name
Inositol oxygenase
Protein Family
UniProt Gene Name
MIOX
UniProt Synonym Gene Names
ALDRL6; KSP32; RSOR; MI oxygenase
UniProt Entry Name
MIOX_HUMAN

Uniprot Description

MIOX: Belongs to the myo-inositol oxygenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.13.99.1; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 22q13.3

Cellular Component: cytoplasm; inclusion body

Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen; oxidoreductase activity, acting on NADH or NADPH; ferric iron binding; aldo-keto reductase activity; inositol oxygenase activity; NADP binding

Biological Process: inositol catabolic process

Research Articles on MIOX

Similar Products

Product Notes

The MIOX miox (Catalog #AAA833848) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MIOX can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IF: 1:100 IHC: 1:50 WB: 1:2000. Researchers should empirically determine the suitability of the MIOX miox for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIOX, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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