Human Lysine Specific Demethylase 6B (KDM6B) ELISA Kit | KDM6B elisa kit

Human Lysine Specific Demethylase 6B (KDM6B) ELISA Kit

Cat. Num. AAA2707702

• Reactivity: Human
• Synonyms: Lysine Specific Demethylase 6B (KDM6B); Human Lysine Specific Demethylase 6B (KDM6B) ELISA Kit; JMJD3; Jumonji Domain Containing 3; Histone Jysine Demethylase; Lysine demethylase 6B; KDM6B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lysine Specific Demethylase 6B (KDM6B).; No significant cross-reactivity or interference between Lysine Specific Demethylase 6B (KDM6B) and analogues was observed.
• Sequence Length: 1645

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.056ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysine Specific Demethylase 6B (KDM6B) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysine Specific Demethylase 6B (KDM6B) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KDM6B elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysine Specific Demethylase 6B (KDM6B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysine Specific Demethylase 6B (KDM6B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysine Specific Demethylase 6B (KDM6B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysine Specific Demethylase 6B (KDM6B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119610532
• UniProt Accession #: O15054
• NCBI Official Full Name: hCG2036579, isoform CRA_a
• UniProt Protein Name: Lysine-specific demethylase 6B
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: KDM6B
• UniProt Synonym Gene Names: JMJD3; KIAA0346
• UniProt Entry Name: KDM6B_HUMAN

Uniprot Description

JMJD3: Histone demethylase that specifically demethylates 'Lys- 27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Interacts with TLE1. Component of the MLL4 complex, at least composed of MLL4, ASH2L, RBBP5, WDR5, and KDM6B. By 12-O-tetradecanoylphorbol-13-acetate (TPA) in myeloid leukemia cells. Belongs to the UTX family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; dioxygenase activity; sequence-specific DNA binding; metal ion binding; beta-catenin binding

Biological Process: endothelial cell differentiation; establishment and/or maintenance of chromatin architecture; mesodermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; cardiac muscle cell differentiation