Immunofluorescence (IF)
(Fig.1. Immunofluorescence staining (1:1000) of V5 tag fusion protein in 293 cells with red and counterstained with DAPI.)
Mouse V5 Tag Monoclonal Antibody | anti-V5 antibody
Anti-V5 Tag Mouse Monoclonal Antibody (11D5)
Applications
Western Blot, Immunofluorescence, Immunoprecipitation
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Synonyms
V5 Tag; Monoclonal Antibody; Anti-V5 Tag Mouse Monoclonal Antibody (11D5); GKPIPNPLLGLDST epitope; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope; anti-V5 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Form/Format
Liquid in PBS, pH7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Applicable Applications for anti-V5 antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:5000
IF: 1:1000
IP: 1:200
IF: 1:1000
IP: 1:200
Tag
V5
Immunogen
Synthetic Peptide
Preparation and Storage
Stable for one year at -20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Immunofluorescence (IF)
(Fig.1. Immunofluorescence staining (1:1000) of V5 tag fusion protein in 293 cells with red and counterstained with DAPI.)
Immunofluorescence (IF)
(Fig.1. Immunofluorescence staining (1:1000) of V5 tag fusion protein in 293 cells with red and counterstained with DAPI.)
Western Blot (WB)
(Fig.2. Western blot analysis of 1ug V5 fusion protein with Anti-V5 mouse monoclonal antibody in 1:5000 (lane A) and 1:10000 (lane B) dilutions. )
Western Blot (WB)
(Fig.2. Western blot analysis of 1ug V5 fusion protein with Anti-V5 mouse monoclonal antibody in 1:5000 (lane A) and 1:10000 (lane B) dilutions. )
Immunoprecipitation (IP)
(Fig.3. IP (1:200)-WB (1:5000) analysis of V5-Tag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with V5-tag protein(lane B); IP untransfected 293 cell lysate with Anti V5 tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti V5 tag mAb (lane E).)
Immunoprecipitation (IP)
(Fig.3. IP (1:200)-WB (1:5000) analysis of V5-Tag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with V5-tag protein(lane B); IP untransfected 293 cell lysate with Anti V5 tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti V5 tag mAb (lane E).)
Related Product Information for anti-V5 antibody
Description: Featured Anti-V5 Tag Mouse Monoclonal Antibody (11D5), specially designed for your taged protein quantification and localization.
Background: The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. This V5-tag antibody is prepared by immunizing synthetic peptide GKPIPNPLLGLDST coupled to KLH.
Background: The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. This V5-tag antibody is prepared by immunizing synthetic peptide GKPIPNPLLGLDST coupled to KLH.
Product Categories/Family for anti-V5 antibody
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Product Notes
The V5 (Catalog #AAA9719157) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's V5 Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:5000 IF: 1:1000 IP: 1:200. Researchers should empirically determine the suitability of the V5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "V5 Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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