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Western Blot (WB) (Western blot analysis using MCAM mAb against human MCAM recombinant protein. (Expected MW is 37.7 kDa))

Mouse anti-Human MCAM Monoclonal Antibody | anti-MCAM antibody

Anti-MCAM Mouse mAb

Gene Names
MCAM; CD146; MUC18
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
MCAM; Monoclonal Antibody; Anti-MCAM Mouse mAb; CD146; MUC18; anti-MCAM antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6C3E6
Form/Format
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
Sequence Length
620
Applicable Applications for anti-MCAM antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Immunogen
Purified recombinant fragment of human MCAM (AA: 84-189) expressed in E Coli.

Western Blot (WB)

(Western blot analysis using MCAM mAb against human MCAM recombinant protein. (Expected MW is 37.7 kDa))

Western Blot (WB) (Western blot analysis using MCAM mAb against human MCAM recombinant protein. (Expected MW is 37.7 kDa))

Western Blot (WB)

(Western blot analysis using MCAM mAb against HEK293 (1) and MCAM (AA)

Western Blot (WB) (Western blot analysis using MCAM mAb against HEK293 (1) and MCAM (AA)

Western Blot (WB)

(Western blot analysis using MCAM mouse mAb against HUVE-12 (1), EVC-304 (2), HELA (3) and MCF-7 (4) cell lysate.)

Western Blot (WB) (Western blot analysis using MCAM mouse mAb against HUVE-12 (1), EVC-304 (2), HELA (3) and MCF-7 (4) cell lysate.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MCF-7 cells using MCAM mouse mAb (green) and negative control (red).)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of MCF-7 cells using MCAM mouse mAb (green) and negative control (red).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MCAM mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MCAM mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MCAM mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MCAM mouse mAb with DAB staining.)
Related Product Information for anti-MCAM antibody
Entrez Summary: The protein encoded by this gene plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
71.6
NCBI Official Full Name
MCAM
NCBI Official Synonym Full Names
melanoma cell adhesion molecule
NCBI Official Symbol
MCAM
NCBI Official Synonym Symbols
CD146; MUC18
NCBI Protein Information
cell surface glycoprotein MUC18
UniProt Protein Name
Cell surface glycoprotein MUC18
Protein Family
UniProt Gene Name
MCAM
UniProt Synonym Gene Names
MUC18

Uniprot Description

MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: external side of plasma membrane; focal adhesion

Biological Process: anatomical structure morphogenesis; angiogenesis; glomerular filtration

Research Articles on MCAM

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Product Notes

The MCAM mcam (Catalog #AAA476416) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-MCAM Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MCAM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the MCAM mcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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