Western Blot (WB)
(Western Blot analysis of MBP-tag fusion protein, using MBP-tag mouse monoclonal antibody.)
Mouse anti-General MBP Monoclonal Antibody | anti-MBP antibody
MBP-Tag Mouse Monoclonal Antibody
Reactivity
General
Applications
Western Blot
Purity
Affinity-chromatography
Synonyms
MBP; Monoclonal Antibody; MBP-Tag Mouse Monoclonal Antibody; anti-MBP antibody
Host
Mouse
Reactivity
General
Clonality
Monoclonal
Isotype
IgG1
Specificity
MBP-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal MBP -tagged recombinant proteins.
Purity/Purification
Affinity-chromatography
Form/Format
Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-MBP antibody
Western Blot (WB)
Application Notes
WB: 1:5000-1:10,000
Immunogen
Synthetic peptide MBP-Tag.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of MBP-tag fusion protein, using MBP-tag mouse monoclonal antibody.)
Western Blot (WB)
(Western Blot analysis of MBP-tag fusion protein, using MBP-tag mouse monoclonal antibody.)
Related Product Information for anti-MBP antibody
MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.
Similar Products
Product Notes
The MBP (Catalog #AAA3014368) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MBP-Tag Mouse Monoclonal Antibody reacts with General and may cross-react with other species as described in the data sheet. AAA Biotech's MBP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:5000-1:10,000. Researchers should empirically determine the suitability of the MBP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MBP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
