Western Blot (WB)
(Western Blot analysis of GST-T7 fusion protein, using T7-tag mouse monoclonal antibody.)
Mouse anti-General T7 Monoclonal Antibody | anti-T7 antibody
T7-Tag Mouse Monoclonal Antibody
Reactivity
General
Applications
Western Blot, Immunofluorescence
Purity
Affinity-chromatography
Synonyms
T7; Monoclonal Antibody; T7-Tag Mouse Monoclonal Antibody; T7 Tag; MASMTGGQQMG epitope tag; Enterobacteria phage T7; Bacteriophage T7; Minor capsid protein 10B; Major capsid protein 10A; anti-T7 antibody
Host
Mouse
Reactivity
General
Clonality
Monoclonal
Isotype
IgG1
Specificity
T7-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal T7- tagged proteins.
Purity/Purification
Affinity-chromatography
Form/Format
Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-T7 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:5000-1:10,000
IF: 1:200
IF: 1:200
Immunogen
Synthetic peptide T7-Tag.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of GST-T7 fusion protein, using T7-tag mouse monoclonal antibody.)
Western Blot (WB)
(Western Blot analysis of GST-T7 fusion protein, using T7-tag mouse monoclonal antibody.)
Related Product Information for anti-T7 antibody
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors that is based on the very efficient T7 RNA polymerase expression system. Antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
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Product Notes
The T7 (Catalog #AAA3014373) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The T7-Tag Mouse Monoclonal Antibody reacts with General and may cross-react with other species as described in the data sheet. AAA Biotech's T7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:5000-1:10,000 IF: 1:200. Researchers should empirically determine the suitability of the T7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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