Mouse anti-Human MBD1 Monoclonal Antibody
MBD1 (Methyl CpG Binding Protein 1, MeCP1, CXXC3, MeCP1 Complex, Methyl CpG Binding Domain Protein 1, Methyl CpG Binding Protein MBD1, Protein Containing Methyl CpG Binding Domain 1, PCM1, Regulator of Fibroblast Growth Factor 2 (FGF 2) Transcription, RFT
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
MBD1; Monoclonal Antibody; MBD1 (Methyl CpG Binding Protein 1; MeCP1; CXXC3; MeCP1 Complex; Methyl CpG Binding Domain Protein 1; Methyl CpG Binding Protein MBD1; Protein Containing Methyl CpG Binding Domain 1; PCM1; Regulator of Fibroblast Growth Factor 2 (FGF 2) Transcription; RFT; Anti -MBD1 (Methyl CpG Binding Protein 1; anti-MBD1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8L709
Specificity
This monoclonal antibody can be used for detection of MBD1, isoform 1, a 605 amino acid protein. In HeLa, a 70kD band is observed. Species Crossreactivity: Human.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Applicable Applications for anti-MBD1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
This antibody was generated by immunizing mice with a synthetic peptide of human MBD1.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-MBD1 antibody
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).
Product Categories/Family for anti-MBD1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The MBD1 (Catalog #AAA600629) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MBD1 (Methyl CpG Binding Protein 1, MeCP1, CXXC3, MeCP1 Complex, Methyl CpG Binding Domain Protein 1, Methyl CpG Binding Protein MBD1, Protein Containing Methyl CpG Binding Domain 1, PCM1, Regulator of Fibroblast Growth Factor 2 (FGF 2) Transcription, RFT reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MBD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the MBD1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MBD1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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