Flow Cytometry (FC/FACS)
(Staining of cultured macrophages from peripheral blood monocytes with 0.25 ug of Mouse IgG1 K Istoty (PE) Control Purified (open histogram) or 0.25 ug of Mature Macrophage Marker antibody (filled histogram) followed by Anti-Mouse IgG (biotin) and Streptavidin (APC). Total viable cells were used for analysis.)
Mouse Mature Macrophage Marker Monoclonal Antibody
Mature Macrophage Marker antibody
Applications
Flow Cytometry, Functional Assay
Purity
Mature Macrophage Marker antibody was purified by affinity chromatography.
Synonyms
Mature Macrophage Marker; Monoclonal Antibody; Mature Macrophage Marker antibody; Monoclonal Mature Macrophage Marker; Anti-Mature Macrophage Marker; anti-Mature Macrophage Marker antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
25F9
Specificity
Human
Purity/Purification
Mature Macrophage Marker antibody was purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-Mature Macrophage Marker antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.0075 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
Biological Significance
The monoclonal antibody 25F9 recognizes a protein on mature macrophages both on the cell surface and in intracellular vesicular structures. Expression is thought to correlate with endocytic macrophages and is useful in many disease models. Expression is not found on immature macrophages or monocytes or any other cell hematopoietic cell.
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of cultured macrophages from peripheral blood monocytes with 0.25 ug of Mouse IgG1 K Istoty (PE) Control Purified (open histogram) or 0.25 ug of Mature Macrophage Marker antibody (filled histogram) followed by Anti-Mouse IgG (biotin) and Streptavidin (APC). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of cultured macrophages from peripheral blood monocytes with 0.25 ug of Mouse IgG1 K Istoty (PE) Control Purified (open histogram) or 0.25 ug of Mature Macrophage Marker antibody (filled histogram) followed by Anti-Mouse IgG (biotin) and Streptavidin (APC). Total viable cells were used for analysis.)
Related Product Information for anti-Mature Macrophage Marker antibody
Mouse monoclonal Mature Macrophage Marker antibody
Product Categories/Family for anti-Mature Macrophage Marker antibody
Similar Products
Product Notes
The Mature Macrophage Marker (Catalog #AAA834952) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Mature Macrophage Marker can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.0075 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. Researchers should empirically determine the suitability of the Mature Macrophage Marker for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mature Macrophage Marker, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
