Rabbit anti-Human, Mouse RARB Polyclonal Antibody | anti-RARB antibody

RARB, NT (RARB, HAP, NR1B2, Retinoic acid receptor beta, HBV-activated protein, Nuclear receptor subfamily 1 group B member 2, RAR-epsilon) (Azide free) (HRP)

Cat. Num. AAA6340332

• Gene Names: RARB; HAP; RRB2; NR1B2; MCOPS12; RARbeta1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RARB; Polyclonal Antibody; NT (RARB; HAP; NR1B2; Retinoic acid receptor beta; HBV-activated protein; Nuclear receptor subfamily 1 group B member 2; RAR-epsilon) (Azide free) (HRP); RAR-epsilon; anti-RARB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 336

• Applicable Applications for anti-RARB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RARB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human RARB.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RARB Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the RARB antibody detected the RARB protein (arrow).)

Related Product Information for anti-RARB antibody

This gene encodes retinoic acid receptor beta, a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. This receptor localizes to the cytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellular signalling in embryonic morphogenesis, cell growth and differentiation. It is thought that this protein limits growth of many cell types by regulating gene expression. The gene was first identified in a hepatocellular carcinoma where it flanks a hepatitis B virus integration site. The gene expresses at least two transcript variants; one additional transcript has been described, but its full length nature has not been determined. [provided by RefSeq].

Product Categories/Family for anti-RARB antibody

Antibodies; Receptors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Miladi-Abdennadher, I., et al. Tumour Biol. 31(5):503-511(2010). Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Ding, Y., et al. Mol. Vis. 16, 855-861 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 7706625
• NCBI GeneID: 5915
• NCBI Accession #: NP_057236.1; NP_000956.2
• NCBI GenBank Nucleotide #: NP_057236.1
• UniProt Accession #: P10826
• NCBI Official Full Name: retinoic acid receptor beta isoform 2
• NCBI Official Synonym Full Names: retinoic acid receptor beta
• NCBI Official Symbol: RARB
• NCBI Official Synonym Symbols: HAP; RRB2; NR1B2; MCOPS12; RARbeta1
• NCBI Protein Information: retinoic acid receptor beta
• UniProt Protein Name: Retinoic acid receptor beta
• Protein Family: Retinoic acid receptor
• UniProt Gene Name: RARB
• UniProt Synonym Gene Names: HAP; NR1B2; RAR-beta
• UniProt Entry Name: RARB_HUMAN

NCBI Description

This gene encodes retinoic acid receptor beta, a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. This receptor localizes to the cytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellular signalling in embryonic morphogenesis, cell growth and differentiation. It is thought that this protein limits growth of many cell types by regulating gene expression. The gene was first identified in a hepatocellular carcinoma where it flanks a hepatitis B virus integration site. Alternate promoter usage and differential splicing result in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

RARB: is a receptor for retinoic acid, a potent mammalian morphogen and teratogen that has profound effects on vertebrate development. RARB is a member of the nuclear receptor superfamily. Controls cell function by directly regulating gene expression. Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. Four splice-variant isoforms have been described. Isoform beta-1 and beta-2 are nuclear and isoform beta-4 cytoplasmic.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor; Oncoprotein

Chromosomal Location of Human Ortholog: 3p24.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; protein binding; DNA binding; zinc ion binding; protein complex binding; steroid hormone receptor activity; drug binding; retinoic acid receptor activity

Biological Process: retinoic acid receptor signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of myelination; striatum development; glandular epithelial cell development; positive regulation of apoptosis; embryonic eye morphogenesis; multicellular organism growth; negative regulation of chondrocyte differentiation; ventricular cardiac muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; signal transduction; embryonic hindlimb morphogenesis; negative regulation of cell proliferation; ureteric bud development; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; gene expression; steroid hormone mediated signaling; positive regulation of neuron differentiation; transmembrane transport; embryonic gut development; negative regulation of apoptosis

Disease: Microphthalmia, Syndromic 12