Western Blot (WB)
(Cell lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Maspin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human Maspin Monoclonal Antibody | anti-SERPINB5 antibody
Maspin antibody
Gene Names
SERPINB5; PI5; maspin
Reactivity
Human
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Maspin antibody was purified by protein-G affinity chromatography
Synonyms
Maspin; Monoclonal Antibody; Maspin antibody; Monoclonal Maspin; Anti-Maspin; Serpin peptidase inhibitor; Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5; member 5; Protease inhibitor 5; Spi7.; SERPINB5; clade B (ovalbumin); Anti Maspin; PI5; Serpin B5; Spi5; clade B; PI-5; Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5 Serine (or cysteine) peptidase inhibitor; Ovalbumin; anti-SERPINB5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT2N6
Specificity
Human
Purity/Purification
Maspin antibody was purified by protein-G affinity chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration
1 mg/ml (varies by lot)
Sequence Length
375
Applicable Applications for anti-SERPINB5 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
WB: 1:1,000-1:2,000
Source Note
Anti-human Maspin antibody, clone AT2N6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Maspin protein.
Biological Significance
Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, also known as Maspin, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion. Also, it is present in normal breast and prostatic epithelial cells but downregulated in the respective carcinomas.
Cross-Reactivity
To be determined by end-user.
Immunogen
Maspin antibody was raised in mouse using recombinant Maspin (1-375aa) purified from E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Cell lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Maspin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(Cell lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Maspin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-SERPINB5 antibody
Mouse monoclonal Maspin antibody
Product Categories/Family for anti-SERPINB5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,763 Da
NCBI Official Full Name
maspin
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade B (ovalbumin), member 5
NCBI Official Symbol
SERPINB5
NCBI Official Synonym Symbols
PI5; maspin
NCBI Protein Information
serpin B5
UniProt Protein Name
Serpin B5
Protein Family
UniProt Gene Name
SERPINB5
UniProt Synonym Gene Names
PI5; PI-5
UniProt Entry Name
SPB5_HUMAN
Similar Products
Product Notes
The SERPINB5 serpinb5 (Catalog #AAA533942) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Maspin antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Maspin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). WB: 1:1,000-1:2,000. Researchers should empirically determine the suitability of the SERPINB5 serpinb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Maspin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
