Mouse anti-Human MAPK9 / JNK2 / SAPK Monoclonal Antibody | anti-MAPK9 antibody

Mouse Monoclonal [clone 1C1-3A8] (IgG1,k) to Human MAPK9 / JNK2 / SAPK

Cat. Num. AAA245799

• Gene Names: MAPK9; JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: MAPK9 / JNK2 / SAPK; Monoclonal Antibody; Mouse Monoclonal [clone 1C1-3A8] (IgG1; k) to Human MAPK9 / JNK2 / SAPK; Anti-MAPK9 / JNK2 / SAPK Antibody (clone 1C1-3A8) IHC-plus; MAPK9; C-Jun kinase 2; C-Jun N-terminal kinase 2; JNK2B; JNK2; JNK2ALPHA; MAP kinase 9; p54a; JNK2BETA; PRKM9; SAPK; MAPK 9; p54aSAPK; SAPK1a; JNK-55; JNK2A; Jun kinase; Human MAPK9; anti-MAPK9 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1C1-3A8
• Specificity: Human JNK2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 424

• Applicable Applications for anti-MAPK9 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot using tissue lysated and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: MAPK9 (AAH32539, 1 a.a. ~ 425 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: MAPK9 / JNK2 / SAPK antibody was raised against mAPK9 (AAH32539, 1 a.a. ~ 425 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MAPK9 / JNK2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of MAPK9 expression in transfected 293T cell line by MAPK9 monoclonal antibody)

RNA interference

(Western blot of MAPK9 over-expressed 293 cell line, cotransfected with MAPK9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK9 monoclonal antibody. GAPDH (36.1 kD) used as specific.)

ELISA (EIA)

(Detection limit for recombinant GST tagged MAPK9 is approximately 3 ng/ml as a capture antibody.)

Related Product Information for anti-MAPK9 antibody

JNK2, a MAPK type protein kinase, regulates cell apoptosis, differentiation and growth in response to growth factors, inflammatory cytokines and cellular stress. JNK2 is phosphorylated by by MKK4/MAP2K4 and MKK7/MAP2K7. Activated JNK2 translocates to the nucleus where it phosphorylates transcription factors, including c-Jun, ATF2, ELK1, Jun-D and NFAT4.

Product Categories/Family for anti-MAPK9 antibody

Family: Protein Kinase; Subfamily: MAPK

NCBI and Uniprot Product Information

• NCBI GI #: 21237736
• NCBI GeneID: 5601
• NCBI Accession #: NP_002743.3
• NCBI GenBank Nucleotide #: NM_002752.4
• UniProt Accession #: P45984; Q15708; Q15710; Q15711; Q8N5C5; A8K0S3; B5BU66; B5M0B4; D3DWQ8; D3DWQ9
• Molecular Weight: 27,334 Da
• NCBI Official Full Name: mitogen-activated protein kinase 9 isoform alpha2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 9
• NCBI Official Symbol: MAPK9
• NCBI Official Synonym Symbols: JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA
• NCBI Protein Information: mitogen-activated protein kinase 9; MAPK 9; Jun kinase; MAP kinase 9; c-Jun kinase 2; c-Jun N-terminal kinase 2; stress-activated protein kinase 1a; stress-activated protein kinase JNK2
• UniProt Protein Name: Mitogen-activated protein kinase 9
• UniProt Gene Name: MAPK9
• UniProt Synonym Gene Names: JNK2; PRKM9; SAPK1A; MAP kinase 9; MAPK 9; SAPK1a
• UniProt Entry Name: MK09_HUMAN

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]

Uniprot Description

JNK2: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses including UV radiation. Phosphorylates specific transcription factors such as c-Jun and ATF2, mediating immediate-early gene expression. Closely related to JNK1. Both are involved in UV radiation induced apoptosis. Blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells in the mouse. Four alternatively-spliced isoforms have been described.

Protein type: Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; CMGC group; MAPK family; MAPK/JNK subfamily; JNK subfamily

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: nucleoplasm; mitochondrion; cytosol

Molecular Function: protein binding; mitogen-activated protein kinase kinase kinase binding; caspase activator activity; JUN kinase activity; transcription factor binding; ATP binding

Biological Process: positive regulation of nitric oxide biosynthetic process; central nervous system development; regulation of protein ubiquitination; response to toxin; positive regulation of transcription, DNA-dependent; rhythmic process; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; regulation of JNK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; JNK cascade; response to stress; toll-like receptor 4 signaling pathway; protein targeting to mitochondrion; neurite development; positive regulation of prostaglandin biosynthetic process; caspase activation; response to drug; release of cytochrome c from mitochondria; MyD88-independent toll-like receptor signaling pathway; regulation of circadian rhythm; positive regulation of chemokine production; JUN phosphorylation; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to cadmium ion; response to mechanical stimulus; positive regulation of prostaglandin secretion; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; positive regulation of protein amino acid phosphorylation; positive regulation of nitric-oxide synthase biosynthetic process; response to amine stimulus