Mouse MAP2K2 Monoclonal Antibody | anti-MAP2K2 antibody

MAP2K2 Antibody

Cat. Num. AAA9602399

• Gene Names: MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA, Flow Cytometry, Functional Assay
• Purity: Affinity-Chromatography
• Synonyms: MAP2K2; Monoclonal Antibody; MAP2K2 Antibody; Cardiofaciocutaneous syndrome; CFC syndrome; CFC4; Dual specificity mitogen activated protein kinase kinase 2; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; FLJ26075; MAP kinase kinase 2; map2k2; MAPK/ERK kinase 2; MAPKK 2; MAPKK2; MEK 2; MEK2; Microtubule associated protein kinase kinase 2; Mitogen activated protein kinase kinase 2; Mitogen activated protein kinase kinase 2 p45; MKK 2; MKK2; MP2K2_HUMAN; OTTHUMP00000165826; OTTHUMP00000165827; PRKMK 2; PRKMK2; anti-MAP2K2 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: MAP2K2 antibody detects endogenous levels of total MAP2K2
• Purity/Purification: Affinity-Chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 400

• Applicable Applications for anti-MAP2K2 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: ELISA: 1:10000; WB: 1:500-1:2000; ICC: 1:200-1:1000; FC/FACS: 1:200-1:400
• Immunogen: Purified recombinant fragment of human MAP2K2 expressed in E. Coli
• Subcellular Location: Cytoplasm, Cytoskeleton, Microtubule Organizing Center, Centrosome. Cytoplasm, Cytoskeleton, Microtubule Organizing Center, Spindle Pole Body. Cytoplasm. Nucleus. Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Figure 1: Western blot analysis using MAP2K2 mouse mAb against PC-12 (1), Jurkat (2), Hela (3) and NIH/3T3 (4) cell lysate.)

Related Product Information for anti-MAP2K2 antibody

Description: MAP2K2, also called MEK2, it is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome.
Function: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
Subunit Structure: Interacts with MORG1 (By similarity). Interacts with SGK1 (PubMed:19447520). Interacts with KSR1 (PubMed:10409742).
Post-translational Modifications: MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity). Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 13489054
• NCBI GeneID: 5605
• NCBI Accession #: NP_109587.1
• NCBI GenBank Nucleotide #: NM_030662.3
• UniProt Accession #: P36507
• Molecular Weight: Observed: 44 kDa; Predicted: 45 kDa
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 2
• NCBI Official Symbol: MAP2K2
• NCBI Official Synonym Symbols: CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 2
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K2
• UniProt Synonym Gene Names: MEK2; MKK2; PRKMK2; MAP kinase kinase 2; MAPKK 2; MEK 2

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases ().