Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human testis stained with MAGE-1 antibody (1:200), peroxidase-conjugate and DAB chromogen.)
Mouse MAGE-1 Monoclonal Antibody | anti-MAGE-1 antibody
MAGE-1 (Melanoma-associated Antigen 1, MAGE-1 Antigen, Cancer/testis Antigen, CTA) (FITC)
Gene Names
MAGEA1; CT1.1; MAGE1
Reactivity
Canine, Human, Rat
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
MAGE-1; Monoclonal Antibody; MAGE-1 (Melanoma-associated Antigen 1; MAGE-1 Antigen; Cancer/testis Antigen; CTA) (FITC); anti-MAGE-1 antibody
Host
Mouse
Reactivity
Canine, Human, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
MA454
Specificity
Recognizes human MAGE-1. Species Crossreactivity: rat and canine.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-MAGE-1 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 1-2ug/500ug protein lysate
IF: 1-2ug/ml
FC/FACS: 0.5-1ug/10e6 cells
Western Blot: 0.5-1ug/ml
Applications are based on unconjugated antibody.
IF: 1-2ug/ml
FC/FACS: 0.5-1ug/10e6 cells
Western Blot: 0.5-1ug/ml
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to full length human MAGE-1.
Conjugate
FITC
Positive Control
Melanoma cell lines. Melanomas, gliomas, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, and renal cell carcinomas.
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human testis stained with MAGE-1 antibody (1:200), peroxidase-conjugate and DAB chromogen.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human testis stained with MAGE-1 antibody (1:200), peroxidase-conjugate and DAB chromogen.)
Product Categories/Family for anti-MAGE-1 antibody
References
1. Ma ZY, Khatlani TS, Sasaki K, Okuda M, Inokuma H, Onishi T. Identification of feline MAGE-1 gene product by monoclonal antibodies. Tissue Antigens. 2002 Aug;60(2):133-8. PMID: 12392507 2. Laduron S, Deplus R, Zhou S, Kholmanskikh O, Godelaine D, De Smet C, Hayward SD, Fuks F, Boon T, De Plaen E. MAGE-A1 interacts with adaptor SKIP and the deacetylase HDAC1 to repress transcription. Nucleic Acids Res. 2004 Aug 17;32(14):4340-50. Print 2004. PMID: 15316101 3. Loriot A, De Plaen E, Boon T, De Smet C. Transient down-regulation of DNMT1 methyltransferase leads to activation and stable hypomethylation of MAGE-A1 in melanoma cells. J Biol Chem. 2006 Apr 14;281(15):10118-26. Epub 2006 Feb 22. PMID: 16497664 4. Pereira CM, Gomes CC, De Fatima Correia Silva J, Bucciarelli Rodriguez M, Barbosa AA, Gomez RS. Evaluation of MAGE A1 in oral squamous cell carcinoma. Oncol Rep. 2012 Jun;27(6):1843-8. doi: 10.3892/or.2012.1736. Epub 2012 Mar 19. PMID: 22447174
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,342 Da
NCBI Official Full Name
melanoma-associated antigen 1
NCBI Official Synonym Full Names
melanoma antigen family A1
NCBI Official Symbol
MAGEA1
NCBI Official Synonym Symbols
CT1.1; MAGE1
NCBI Protein Information
melanoma-associated antigen 1; MAGE-1 antigen; antigen MZ2-E; cancer/testis antigen 1.1; cancer/testis antigen family 1, member 1; melanoma antigen MAGE-1; melanoma antigen family A 1; melanoma antigen family A, 1 (directs expression of antigen MZ2-E); me
UniProt Protein Name
Melanoma-associated antigen 1
UniProt Gene Name
MAGEA1
UniProt Synonym Gene Names
MAGE1; MAGE1A; CT1.1
UniProt Entry Name
MAGA1_HUMAN
Uniprot Description
MAGE-A1: a tumor antigen expressed in a variety of tumor cells and in the testis. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
Protein type: Cell surface
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: cytoplasm; plasma membrane; nucleus
Molecular Function: protein binding; histone deacetylase binding
Biological Process: transcription, DNA-dependent; negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter
Similar Products
Product Notes
The MAGE-1 magea1 (Catalog #AAA6122324) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MAGE-1 (Melanoma-associated Antigen 1, MAGE-1 Antigen, Cancer/testis Antigen, CTA) (FITC) reacts with Canine, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAGE-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). IP: 1-2ug/500ug protein lysate IF: 1-2ug/ml FC/FACS: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MAGE-1 magea1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAGE-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
