Mouse Macrophages mature Monoclonal Antibody
Macrophages mature, Human, mAb 25F9, Biotin
Reactivity
Reactivity: Human. Cross reactivity: Rhesus monkey
Applications
Flow Cytometry, Functional Assay, Western Blot
Synonyms
Macrophages mature; Monoclonal Antibody; Human; mAb 25F9; Biotin; anti-Macrophages mature antibody
Host
Mouse
Reactivity
Reactivity: Human. Cross reactivity: Rhesus monkey
Clonality
Monoclonal
Isotype
Mouse IgG1
Form/Format
0.5 ml (100 ug/ml) 0.2 um filtered biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Applicable Applications for anti-Macrophages mature antibody
F, Flow Cytometry (FC/FACS), P, Western Blot (WB
Application Notes
Application Use: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Conjugate
Biotin
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-Macrophages mature antibody
Product Description: The monoclonal antibody 25F9 recognises a protein of 86 kD on the cell surface and within the cytoplasm of mature macrophages. The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. Furthermore the monoclonal antibody 25F9 cross reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells. The monoclonal antibody 25F9 is very useful for macrophage phenotyping.
References
1. Rosseau, S et al; Phenotypic characterization of alveolar monocyte recruitment in acute respiratory distress syndrome. Am J Physiol Lung Cell Mol Physiol 2000, 279: L25 2. Zwadlo, G et al; A monoclonal antibody to a differentiation antigen present on human macrophages and absent from monocytes. J Immunol 1985, 134: 1487
3. Kiefer, R et al; Macrophage differentiation antigens in acute and chronic autoimmune polyneuropathies. Brain 1998, 121: 469
4. Zwadlo, G et al; A monoclonal antibody to a novel differentiation antigen on human macrophages associated with the down-regulatory phase of the inflammatory process. Exp Cell Biol 1987, 55: 295
3. Kiefer, R et al; Macrophage differentiation antigens in acute and chronic autoimmune polyneuropathies. Brain 1998, 121: 469
4. Zwadlo, G et al; A monoclonal antibody to a novel differentiation antigen on human macrophages associated with the down-regulatory phase of the inflammatory process. Exp Cell Biol 1987, 55: 295
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Product Notes
The Macrophages mature (Catalog #AAA584286) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Macrophages mature, Human, mAb 25F9, Biotin reacts with Reactivity: Human. Cross reactivity: Rhesus monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Macrophages mature can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), P, Western Blot (WB. Application Use: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the Macrophages mature for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Macrophages mature, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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