Monkey Procalcitonin ELISA Kit | PCT elisa kit
Monkey Procalcitonin ELISA Kit
Reactivity
Monkey
Synonyms
Procalcitonin; Monkey Procalcitonin ELISA Kit; PCT elisa kit
Reactivity
Monkey
Specificity
This assay has high sensitivity and excellent specificity for detection of PCT. No significant cross-reactivity or interference between PCT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PCT and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PCT elisa kit
Intended Uses: This PCT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey PCT. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: PCT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PCT antibody and an PCT-HRP conjugate. The assay sample and buffer are incubated together with PCT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCT concentration since PCT from samples and PCT-HRP conjugate compete for the anti-PCT antibody binding site. Since the number of sites is limited, as more sites are occupied by PCT from the sample, fewer sites are left to bind PCT-HRP conjugate. A standard curve is p lotted relating the intensity of the color (O.D.) to the concentration of standards. The PCT concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PCT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PCT antibody and an PCT-HRP conjugate. The assay sample and buffer are incubated together with PCT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCT concentration since PCT from samples and PCT-HRP conjugate compete for the anti-PCT antibody binding site. Since the number of sites is limited, as more sites are occupied by PCT from the sample, fewer sites are left to bind PCT-HRP conjugate. A standard curve is p lotted relating the intensity of the color (O.D.) to the concentration of standards. The PCT concentration in each sample is interpolated from this standard curve.
Product Categories/Family for PCT elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,733 Da
NCBI Official Full Name
propionate CoA-transferase Pct
NCBI Official Symbol
pct
NCBI Protein Information
propionate CoA-transferase Pct
UniProt Protein Name
Propionate CoA-transferase Pct
UniProt Gene Name
pct
UniProt Synonym Gene Names
RLO149_c008140
UniProt Entry Name
F7ZLI1_ROSLO
