Flow Cytometry (FC/FACS)
(Staining of LYVE-1 transfected cells with 0.06 ug of Rat IgG1 kappa Isotype Control (biotin) (blue histogram) or 0.06 ug of Anti-Mouse Lyve-1 (biotin) (purple histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Rat LYVE1 Monoclonal Antibody | anti-LYVE1 antibody
LYVE1 antibody (biotin)
Applications
Flow Cytometry, Functional Assay
Synonyms
LYVE1; Monoclonal Antibody; LYVE1 antibody (biotin); Monoclonal LYVE1; Anti-LYVE1; Hyaluronic acid receptor; Lymphatic Vessel Endothelial Hyaluronan Receptor 1; Cell surface retention sequence-binding protein 1; LYVE-1; LYVE 1; CRSBP-1; Extracellular link domain-containing protein 1; anti-LYVE1 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ALY7
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
322
Applicable Applications for anti-LYVE1 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.5 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein with similarity to CD44. The extracellular domain contains a conserved hyaluronan binding domain also found in CD44. Expression is found on lymphatic and liver endothelial cells and some populations of macrophages. The lymphatic system is responsible for transporting proteins and cells to tissues throughout the body, thereby acting as immune surveyors. LYVE-1 is one characteristic protein, along with podoplanin, PROX-1, Tie-2 and VEGFR-3, that is expressed on lymphatic endothelial cells. The ligand for LYVE-1 is hyaluronan, a large mucopolysaccharide. Although LYVE-1 can bind hyaluronan in vitro, the site for ligand binding in vivo is masked by sialyated O-linked glycan chains. It is postulated that binding to ligand requires modification/unmasking to expose the binding site. The development and remodeling of the endothelium after injury is an area of extensive study. When transplanted, hematopoietic stem cells can give rise to LECs that integrate into the endothelium in normal and metastatic tissue.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of LYVE-1 transfected cells with 0.06 ug of Rat IgG1 kappa Isotype Control (biotin) (blue histogram) or 0.06 ug of Anti-Mouse Lyve-1 (biotin) (purple histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of LYVE-1 transfected cells with 0.06 ug of Rat IgG1 kappa Isotype Control (biotin) (blue histogram) or 0.06 ug of Anti-Mouse Lyve-1 (biotin) (purple histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Related Product Information for anti-LYVE1 antibody
Rat monoclonal LYVE1 antibody (biotin)
Product Categories/Family for anti-LYVE1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
LYVE1, partial
Protein Family
Similar Products
Product Notes
The LYVE1 (Catalog #AAA834097) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's LYVE1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.5 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the LYVE1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LYVE1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
