Immunohistochemistry (IHC)
(Anti-Osteocalcin Picoband antibody, MBS176906-1.JPGIHC(P): Human Osteosarcoma Tissue )
Rabbit anti-Human Osteocalcin Polyclonal Antibody | anti-BGLAP antibody
Anti-Osteocalcin Antibody
IHC-P: 0.5-1ug/ml
WB: The detection limit for Osteocalcin is approximately 0.25ng/lane under reducing conditions.
Tested Species: In-house tested species with positive test results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunohistochemistry (IHC)
(Anti-Osteocalcin Picoband antibody, MBS176906-1.JPGIHC(P): Human Osteosarcoma Tissue )
Immunohistochemistry (IHC)
(Anti-Osteocalcin Picoband antibody, MBS176906-1.JPGIHC(P): Human Osteosarcoma Tissue )
Western Blot (WB)
(Anti-Osteocalcin Picoband antibody, MBS176906-2.jpgAll lanes: Anti-Osteocalcin(MBS176906) at 0.5ug/mlWB: Recombinant Human Osteocalcin Protein 0.5ngPredicted bind size: 38KDObserved bind size: 38KD )
Western Blot (WB)
(Anti-Osteocalcin Picoband antibody, MBS176906-2.jpgAll lanes: Anti-Osteocalcin(MBS176906) at 0.5ug/mlWB: Recombinant Human Osteocalcin Protein 0.5ngPredicted bind size: 38KDObserved bind size: 38KD )
Background: Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein found in bone and dentin. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A. It is mapped to 1q22. Osteocalcin may play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building. Osteocalcin acts as a hormone in the body, causing beta cellsin the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. It may play a role in male fertility. It is found that picomolar amounts of osteocalcin affected insulin secretion and beta-cell proliferation.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
Uniprot Description
osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family.
Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; dendrite; cytoplasm; perikaryon
Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity
Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; osteoblast differentiation; odontogenesis; response to vitamin D; regulation of osteoclast differentiation; response to ethanol; response to vitamin K; response to zinc ion; response to mechanical stimulus; response to estrogen stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; skeletal development; regulation of bone mineralization
Research Articles on BGLAP
Similar Products
Product Notes
The BGLAP bglap (Catalog #AAA176906) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Osteocalcin Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Osteocalcin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: 0.1-0.5ug/ml IHC-P: 0.5-1ug/ml WB: The detection limit for Osteocalcin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive test results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the BGLAP bglap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Osteocalcin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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