Flow Cytometry (FC/FACS)
(Staining of Guinea Pig peripheral blood lymphocytes with Mouse anti Guinea Pig Lymphocytes and Langerhans Cells followed by Goat anti Mouse IgG (H/L):FITC )
Mouse anti-Guinea Pig LYMPHOCYTES AND LANGERHANS CELLS Monoclonal Antibody
MOUSE ANTI GUINEA PIG LYMPHOCYTES AND LANGERHANS CELLS
Reactivity
Guinea Pig
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
LYMPHOCYTES AND LANGERHANS CELLS; Monoclonal Antibody; MOUSE ANTI GUINEA PIG LYMPHOCYTES AND LANGERHANS CELLS; anti-LYMPHOCYTES AND LANGERHANS CELLS antibody
Host
Mouse
Reactivity
Guinea Pig
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MsGp2
Specificity
LYMPHOCYTES
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-LYMPHOCYTES AND LANGERHANS CELLS antibody
Immunohistochemistry (IHC) Formalin, Flow Cytometry (FC/FACS)
Application Notes
Flow Cytometry: 1/50; 1/200
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Store at +4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch
Shelf Life: 18 months from date of despatch
Flow Cytometry (FC/FACS)
(Staining of Guinea Pig peripheral blood lymphocytes with Mouse anti Guinea Pig Lymphocytes and Langerhans Cells followed by Goat anti Mouse IgG (H/L):FITC )
Flow Cytometry (FC/FACS)
(Staining of Guinea Pig peripheral blood lymphocytes with Mouse anti Guinea Pig Lymphocytes and Langerhans Cells followed by Goat anti Mouse IgG (H/L):FITC )
Testing Data
(Published customer image: Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 used for the detection of infiltrating lymphocytes in guinea pig skin following intradermal Mantoux injection and subsequent electroporation by immunofluorescence.Image caption:Histological analysis reveals infiltration of lymphocytes at the EP treatment site. Guinea pig skin sections were immunohistochemically stained with an antibody recognizing lymphocytes and Langerhans cells (Tetramethylrhodamine isothiocyanate (TRITC) secondary) after ID GFP plasmid administration followed by EP with SEP. Skin biopsies were removed, cryosectioned, antibody and 4',6-diamidino-2-phenylindole (DAPI) stained and visualized under fluorescence microscopy at 20x and 40x. An untreated control (no DNA, no EP) is also shown.From: Mendoza, J.M.; Amante, D.H.; Kichaev, G.; Knott, C.L.; Kiosses, W.B.; Smith, T.R.F.; Sardesai, N.Y.; Broderick, K.E.Elucidating the Kinetics of Expression and Immune Cell Infiltration Resulting from Plasmid Gene Delivery Enhanced by Surface Dermal Electroporation. Vaccines 2013, 1, 384-397.)
Testing Data
(Published customer image: Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 used for the detection of infiltrating lymphocytes in guinea pig skin following intradermal Mantoux injection and subsequent electroporation by immunofluorescence.Image caption:Histological analysis reveals infiltration of lymphocytes at the EP treatment site. Guinea pig skin sections were immunohistochemically stained with an antibody recognizing lymphocytes and Langerhans cells (Tetramethylrhodamine isothiocyanate (TRITC) secondary) after ID GFP plasmid administration followed by EP with SEP. Skin biopsies were removed, cryosectioned, antibody and 4',6-diamidino-2-phenylindole (DAPI) stained and visualized under fluorescence microscopy at 20x and 40x. An untreated control (no DNA, no EP) is also shown.From: Mendoza, J.M.; Amante, D.H.; Kichaev, G.; Knott, C.L.; Kiosses, W.B.; Smith, T.R.F.; Sardesai, N.Y.; Broderick, K.E.Elucidating the Kinetics of Expression and Immune Cell Infiltration Resulting from Plasmid Gene Delivery Enhanced by Surface Dermal Electroporation. Vaccines 2013, 1, 384-397.)
Related Product Information for anti-LYMPHOCYTES AND LANGERHANS CELLS antibody
Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 recognizes an antigen present on the majority of T and B lymphocytes and thymocytes, although the antigen is not expressed by germinal centre cells of the lymph node (Kraal et al 1986). Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 also reacts strongly with epidermal Langerhans cells, giving a superior profile than anti-MHC Class II antibodies on these cells (Healey et al. 1987). Clone MsGp2 has the same distribution as Mouse anti Guinea Pig CD90 antibody, clone CT4 which detects an antigen reportedly involved in the organ non-specific trafficking of guinea pig lymphocytes (Kraal et al 1988). However, unlike clone CT4, clone MsGp2 does not functionally inhibit lymphocyte adherence to high endothelial venules in vitro (Healey et al. 1987).
Similar Products
Product Notes
The LYMPHOCYTES AND LANGERHANS CELLS (Catalog #AAA224794) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI GUINEA PIG LYMPHOCYTES AND LANGERHANS CELLS reacts with Guinea Pig and may cross-react with other species as described in the data sheet. AAA Biotech's LYMPHOCYTES AND LANGERHANS CELLS can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin, Flow Cytometry (FC/FACS). Flow Cytometry: 1/50; 1/200. Researchers should empirically determine the suitability of the LYMPHOCYTES AND LANGERHANS CELLS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LYMPHOCYTES AND LANGERHANS CELLS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
