Ly6G recombinant antibody
Anti-Ly6G [1A8]
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A Affinity Purified
Synonyms
Ly6G; Monoclonal Recombinant Antibody; Anti-Ly6G [1A8]; Ly-6G; GR-1 ; GR1; Lymphocyte antigen 6G; Ly6G recombinant antibody
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, Kappa
Clone Number
1A8
Specificity
Recognizes the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C.
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
111
Applicable Applications for Ly6G recombinant antibody
Flow Cytometry (FC/FACS), Depletion
Application Notes
Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+).
Immunogen
LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells
Buffer Composition
PBS only with 0.02% Proclin 300.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Related Product Information for Ly6G recombinant antibody
Recombinant monoclonal antibody to Ly6G. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1A8.
LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells
LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells
Product Categories/Family for Ly6G recombinant antibody
References
Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. Journal of Immunology PMID:8360469
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
11,798 Da
NCBI Official Full Name
Lymphocyte antigen 6G
UniProt Protein Name
Lymphocyte antigen 6G
Protein Family
UniProt Gene Name
Ly6g
UniProt Synonym Gene Names
Ly-6G
Similar Products
Product Notes
The Ly6G ly6g (Catalog #AAA488299) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ly6G [1A8] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ly6G can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Depletion. Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+). Researchers should empirically determine the suitability of the Ly6G ly6g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ly6G, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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