Mouse LOX Monoclonal Antibody | anti-LOX antibody

LOX antibody

Cat. Num. AAA833771

• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: LOX antibody was purified by affinity chromatography.
• Synonyms: LOX; Monoclonal Antibody; LOX antibody; Monoclonal LOX; Anti-LOX; Lysyl oxidase; MGC105112; Protein lysine 6 oxidase; anti-LOX antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10D11
• Purity/Purification: LOX antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 417

• Applicable Applications for anti-LOX antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC: 1:100; IHC: 1:150; WB: 1:1000
• Biological Significance: LOX is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, The encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutislaxa type I (CL type I).
• Cross-Reactivity: Human
• Immunogen: LOX antibody was raised in mouse using a human recombinant protein fragment corresponding to amino acids 22-168 of human LOX (NP_002308) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant LOX protein (Right) or empty vector (Left) detected with LOX antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of LOX protein in paraffin embedded Adenocarcinoma of Human colon tissue using LOX antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant LOX protein using LOX antibody)

Related Product Information for anti-LOX antibody

Mouse monoclonal LOX antibody

Product Categories/Family for anti-LOX antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 20149540
• NCBI GeneID: 4015
• NCBI Accession #: NP_002308
• NCBI GenBank Nucleotide #: NM_002317.5
• UniProt Accession #: P28300; Q5FWF0; B2R5Q3
• Molecular Weight: 46,944 Da
• NCBI Official Full Name: protein-lysine 6-oxidase isoform 1 preproprotein
• NCBI Official Synonym Full Names: lysyl oxidase
• NCBI Official Symbol: LOX
• NCBI Protein Information: protein-lysine 6-oxidase
• UniProt Protein Name: Protein-lysine 6-oxidase
• Protein Family: Linoleate 9/13-lipoxygenase
• UniProt Gene Name: LOX
• UniProt Entry Name: LYOX_HUMAN

NCBI Description

The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.

Protein type: Secreted; EC 1.4.3.13; Oxidoreductase; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: extracellular space; proteinaceous extracellular matrix; collagen; extracellular region; nucleus

Molecular Function: protein binding; copper ion binding; protein-lysine 6-oxidase activity

Biological Process: response to drug; elastic fiber assembly; blood vessel development; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; response to steroid hormone stimulus; protein modification process; lung development

Disease: Cutis Laxa, Autosomal Recessive, Type Ia