Human Lysyl Oxidase ELISA Kit | LOX elisa kit

Human Lysyl Oxidase ELISA Kit

Cat. Num. AAA766044

• Reactivity: Human
• Synonyms: Lysyl Oxidase; Human Lysyl Oxidase ELISA Kit; LOX/Protein-Lysine 6-Oxidase/MGC105112/Lysyl oxidase; LOX elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LOX. No significant cross-reactivity or interference between LOX and analogues was observed.
• Sequence Length: 417

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 0.625-40ng/ml
• Sensitivity: <0.375ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LOX were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LOX were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LOX elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- LOX antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- LOX antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LOX amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LOX can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 257065
• NCBI GeneID: 4015
• Molecular Weight: 46,944 Da
• NCBI Official Full Name: lysyl oxidase
• NCBI Official Synonym Full Names: lysyl oxidase
• NCBI Official Symbol: LOX
• NCBI Protein Information: protein-lysine 6-oxidase
• UniProt Protein Name: Protein-lysine 6-oxidase
• Protein Family: Lysyl oxidase
• UniProt Gene Name: LOX
• UniProt Entry Name: LYOX_HUMAN

NCBI Description

This gene encodes a member of the lysyl oxidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a regulatory propeptide and the mature enzyme. The copper-dependent amine oxidase activity of this enzyme functions in the crosslinking of collagens and elastin, while the propeptide may play a role in tumor suppression. [provided by RefSeq, Nov 2015]

Uniprot Description

LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.

Protein type: Oxidoreductase; Secreted; EC 1.4.3.13; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: collagen; extracellular region; extracellular space; nucleus; proteinaceous extracellular matrix

Molecular Function: copper ion binding; protein binding; protein-lysine 6-oxidase activity

Biological Process: collagen fibril organization; elastic fiber assembly; extracellular matrix organization and biogenesis; heart development; lung development; protein modification process; response to drug; response to steroid hormone stimulus; wound healing

Disease: Cutis Laxa, Autosomal Recessive, Type Ia