Mouse LMNB1 Monoclonal Antibody | anti-LMNB1 antibody

LMNB1 (Lamin B1, ADLD, LMN, LMN2, LMNB, MGC111419) (HRP)

Cat. Num. AAA6182371

• Gene Names: LMNB1; LMN; ADLD; LMN2; LMNB
• Applications: Immunofluorescence, Immunoprecipitation, Western Blot
• Purity: Purified
• Synonyms: LMNB1; Monoclonal Antibody; LMNB1 (Lamin B1; ADLD; LMN; LMN2; LMNB; MGC111419) (HRP); Lamin B1; MGC111419; anti-LMNB1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4B10
• Specificity: Recognizes LMNB1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-LMNB1 antibody: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: LMNB1 (NP_005564.1, 107aa-186aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Jurkat (Cat # L017V1).)

Western Blot (WB)

(LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in NIH/3T3.)

Western Blot (WB)

(LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Raw 264.7.)

Western Blot (WB)

(Western Blot analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate (66.4 KDa).Lane 2: Non-transfected lysate.)

Immunoprecipitation (IP)

(Immunoprecipitation of LMNB1 transfected lysate using anti-LMNB1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LMNB1 MaxPab rabbit polyclonal antibody.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to LMNB1 on HeLa cell. [antibody concentration 10 ug/ml])

Product Categories/Family for anti-LMNB1 antibody

Antibodies; Lamin; Laminin

NCBI and Uniprot Product Information

• NCBI GI #: 5031877
• NCBI GeneID: 4001
• NCBI Accession #: NP_005564.1
• NCBI GenBank Nucleotide #: NM_005573.3
• UniProt Accession #: P20700; Q3SYN7; Q96EI6; B2R6J6
• Molecular Weight: 66,408 Da
• NCBI Official Full Name: lamin-B1 isoform 1
• NCBI Official Synonym Full Names: lamin B1
• NCBI Official Symbol: LMNB1
• NCBI Official Synonym Symbols: LMN; ADLD; LMN2; LMNB
• NCBI Protein Information: lamin-B1
• UniProt Protein Name: Lamin-B1
• Protein Family: Lamin
• UniProt Gene Name: LMNB1
• UniProt Synonym Gene Names: LMN2; LMNB
• UniProt Entry Name: LMNB1_HUMAN

NCBI Description

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: nucleoplasm; nuclear membrane; membrane; lamin filament; nuclear inner membrane; nuclear envelope

Molecular Function: phospholipase binding; structural molecule activity

Biological Process: apoptosis; cell structure disassembly during apoptosis

Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant