Mouse Lipoteichoic Acid Monoclonal Antibody

Lipoteichoic Acid, mAb 55

Cat. Num. AAA584232

• Applications: Immunofluorescence, Flow Cytometry, Western Blot
• Synonyms: Lipoteichoic Acid; Monoclonal Antibody; mAb 55; ATA; anti-Lipoteichoic Acid antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG3
• Form/Format: Culture medium+0.02%NaN₃; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Concentration: >200 ug/mL (varies by lot)

• Applicable Applications for anti-Lipoteichoic Acid antibody: Immunofluorescence (IF), Flow Cytometry (FC), Immuno Assays (IA), Western Blot (WB)
• Application Notes: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.; ; IA: For detection, 1.2 ug/ml antibody in PBS was added for 1 hr at 37 degree C on LTA coated plates (Ref. 2,3,8).; IF: 60’in PBS/0.02%BSA/0.02% saponin (ref 4); W: A reduced or native sample treatment and run on 15% SDS-Page. Blot was incubated o/n at 4°C with a 1/1000 dilution. The band size is ~17 kDa (Ref.6,7).; Positive Control: Culture medium of Gram-positive bacteria; Negative Control: Culture medium of eukaryotic cells
• Immunogen: Microbial mixture of Streptococcus sobrims HG961, HG962, HG970, and HG977
• Positive Control: Culture medium of Gram-positive bacteria.
• Preparation and Storage: Store at 4°C. Stable for up to 1 year.

Test Data

Related Product Information for anti-Lipoteichoic Acid antibody

Product Description: The monoclonal antibody 55 recognizes lipoteichoic acid (LTA). LTA, a glycerol phosphate surface polymer, is a component of the envelope of Gram-positive bacteria. LTA is anchored via its glycolipids to the membrane and carries a polysaccharide chain extending into the peptidoglycan layer of the cell wall. LTA is released spontaneously into the culture medium during growth of gram-positive bacteria. LTA functions as an immune activator with characteristics very similar to lipopolysaccharide (LPS) from Gram-negative bacteria. LTA binds to CD14 and triggers activation predominantly via Toll-like receptor 2.- Although LTA is internalized and traffics to the Golgi, the cellular activation in response to LTA occurs at the cell surface.

References

1. Hogg,S et al; Occurrence of lipotechoic acid in oral streptococci. Int. journal of systematic bacteriology 1997, 47:62 2. Langevelde, P et al; Antibiotic-induced release of lipoteichoic acid and peptidoglycan from Staphylococcus aureus: Quantitative measurements and biological reactivities. Antimicrob Agents Chemother 1998, 42: 3073
3. Langevelde, P et al; Antibiotic-induced cell wall fragments of Staphylococcus aureus increase endothelial chemokine secretion and adhesiveness for granulocytes. Antimicrob Agents Chemother 1999, 43: 2984
4. Triantafilou, M et al; Lipoteichoic acid and toll-like receptor 2 internalization and targeting to the golgi are lipid raft-dependent. J Biol Chem 2004, 279: 40882
5. Henneke, P et al; Role of lipoteichoic acid in the phagocyte response to group B Streptococcus. J Immunol 2005, 174: 6449
6. Grundling, A et al. Synthesis of glycerol phosphate lipoteichoic acid in Staphylococcus aureus. PNAS 2007, 104:8478
7. Jimenez-Dalmaroni, M et al. Soluble CD36 ectodomain binds negatively charged diacylglycerol ligands and acts as a co-receptor for TLR2. Plos One 2009, 4:7411
8. Yajima, A et al. Contribution of phosphoglucosamine mutase to the resistance of Streptococcus gordonii DL1 to polymorphonuclear leukocyte killing. FEMS Microbiol lett 2009, 297: 196
9. Hashimoto, Y et al. Identification of LTA as a ligand for draper in the phagocytosis of Staphylococcus aureus by Drosophila hemocytes. I.Immunol 2009, 183:7451
10. Hirose, Y et al. Lipoteichoic acids on lactobacillus platarum cell surfaces correlate with induction of interleukin-12p40 production. Microbiol immunol 2010, 54:143