Mouse LIPOPROTEIN LIPASE Monoclonal Antibody | anti-LPL antibody

MOUSE ANTI LIPOPROTEIN LIPASE:Low Endotoxin

Cat. Num. AAA224502

• Applications: ELISA, Functional Assay, Immunoprecipitation, Western Blot
• Synonyms: LIPOPROTEIN LIPASE; Monoclonal Antibody; MOUSE ANTI LIPOPROTEIN LIPASE:Low Endotoxin; anti-LPL antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5D2
• Form/Format: Low Endotoxin; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 478

• Applicable Applications for anti-LPL antibody: ELISA (EIA), Functional Assays (FN), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.; ELISA: Minimum Dilution: 1/500; Maximum Dilution: 1/5000
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Immunogen: Purified bovine milk lipoprotein lipase; Buffer Solution
• Target Species: Bovine
• Preparation and Storage: Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Bovine lipoprotein lipase detected with Mouse anti lipoprotein lipase (MBS224619) followed by Rabbit F(ab')2 anti Mouse IgG:HRP (MBS235192))

Testing Data #2

(Bovine lipoprotein lipase detected with Mouse anti lipoprotein lipase:HRP (MBS224481) )

Related Product Information for anti-LPL antibody

Mouse anti Lipoprotein Lipase antibody, clone 5D2 specifically recognizes an epitope within amino acids 380-410 of lipoprotein lipase (LPL), a member of the AB hydrolase superfamily, which plays a pivotal role in lipoprotein metabolism and transport, acting as the key enzyme in the hydrolysis of triglycerides and very low density lipoproteins (VLDLs), and the release of free fatty acids into peripheral tissues. A deficiency of LPL can result in hypertriglyceridemia, and many studies have focused on the critical role which LPL plays in the pathogenesis of atherosclerosis, and in particular the relationship between LPL and apolipoprotein E (ApoE), both of which are secreted in significant amounts by macrophages in developing arterial wall lesions. Mouse anti Lipoprotein Lipase antibody, clone 5D2 is a unique antibody which differentiates between monomeric inactive and dimeric active LPL, and binds to LPL sequences involved in LPL, LPL receptor, and heparin interactions.  Mouse anti Lipoprotein Lipase antibody, clone 5D2 has been shown to inhibit the activity of human LPL (Chang et al. 1998).

NCBI and Uniprot Product Information

• NCBI GI #: 115497164
• NCBI GeneID: 280843
• NCBI Accession #: NP_001068588.1
• NCBI GenBank Nucleotide #: NM_001075120.1
• UniProt Accession #: P11151; Q17R03
• Molecular Weight: 53,378 Da
• NCBI Official Full Name: lipoprotein lipase
• NCBI Official Synonym Full Names: lipoprotein lipase<
• NCBI Official Symbol: LPL
• NCBI Protein Information: lipoprotein lipase
• UniProt Protein Name: Lipoprotein lipase
• Protein Family: Lipoprotein
• UniProt Gene Name: LPL
• UniProt Synonym Gene Names: LPL
• UniProt Entry Name: LIPL_BOVIN

Uniprot Description

The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.