Testing Data
(Fig.1: FACS analysis of BOSC23 cells using BBX-3A8 Cat.# MBS468030. BOSC23 cells were transiently transfected with an expression vector encoding either LGR4-hum (red curve), LGR4-murine (blue curve) or an irrelevant protein (control transfectant: black curve). Binding of BBX-3A8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained with LGR4- hum and LGR4-mur transfected cells.)
Rat anti-Human, Mouse LGR4 Monoclonal Antibody | anti-LGR4 antibody
Anti-LGR4 (GPR48) monoclonal antibody
CELISA:1:200 - 1:400
For each application a titration should be performed to determined the optimal concentration.
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using BBX-3A8 Cat.# MBS468030. BOSC23 cells were transiently transfected with an expression vector encoding either LGR4-hum (red curve), LGR4-murine (blue curve) or an irrelevant protein (control transfectant: black curve). Binding of BBX-3A8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained with LGR4- hum and LGR4-mur transfected cells.)
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using BBX-3A8 Cat.# MBS468030. BOSC23 cells were transiently transfected with an expression vector encoding either LGR4-hum (red curve), LGR4-murine (blue curve) or an irrelevant protein (control transfectant: black curve). Binding of BBX-3A8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained with LGR4- hum and LGR4-mur transfected cells.)
SDS-PAGE
(SDS-PAGE analysis purifed BBX-3A8 monoclonal antibody.Lane 1: molecular weight marker Lane 2: 2ug of purified BBX-3A8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
SDS-PAGE
(SDS-PAGE analysis purifed BBX-3A8 monoclonal antibody.Lane 1: molecular weight marker Lane 2: 2ug of purified BBX-3A8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
LGR4 (Leucine-rich repeat- containing G protein-coupled receptor 4) belongs to the superfamily of G protein-coupled receptors (GPCRs). It is a 951 amino acid multi-pass membrane protein and has multiple N-terminal leucine-rich repeats, which are important for interaction with the glycoprotein ligands, and 7 transmembrane domains (1,3). LGR4 is highly expressed in the adult human pancreas but also with moderate levels of expression in placenta, kidney, brain and heart (2). LGR4 functions as an orphan receptor that may be involved in physiologic activities throughout the cell. It is overexpressed in various cancer types and is thought to enhance carcinoma invasiveness and metastasis, suggesting an important role in tumor progression.
Loh ED, Broussard SR, Liu Q, Copeland NG, Gilbert DJ, Jenkins NA, Kolakowski, LF Jr.(2000). Chromosomal localization of GPR48, a novel glycoprotein hormone receptor like GPCR, in human and mouse with radiation hybrid and interspecific backcross mapping. Cytogenet Cell Genet. 89(1-2):2-5
Loh ED, Broussard SR, Kolakowski LF (2001). Molecular characterization of a novel glycoprotein hormone G-protein-coupled receptor. Biochem Biophys Res Commun. 282(3):757-64
NCBI and Uniprot Product Information
NCBI Description
G protein-coupled receptors (GPCRs) play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain (Weng et al., 2008 [PubMed 18424556]).[supplied by OMIM, Aug 2008]
Uniprot Description
LGR4: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Plays an essential role during homeostasis of the adult mouse intestine.
Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR
Chromosomal Location of Human Ortholog: 11p14-p13
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity
Biological Process: osteoblast differentiation; G-protein coupled receptor protein signaling pathway; epithelial cell proliferation; positive regulation of transcription, DNA-dependent; negative regulation of cytokine secretion; negative regulation of toll-like receptor signaling pathway; male genitalia development; innate immune response; spermatogenesis; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; bone remodeling; bone mineralization
Disease: Bone Mineral Density Quantitative Trait Locus 17
Research Articles on LGR4
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Product Notes
The LGR4 lgr4 (Catalog #AAA468030) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-LGR4 (GPR48) monoclonal antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LGR4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), CELISA. Flow Cytometry:1.2 ug/106cells CELISA:1:200 - 1:400 For each application a titration should be performed to determined the optimal concentration. Researchers should empirically determine the suitability of the LGR4 lgr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LGR4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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