Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa LGI1 protein using Mouse Anti-LGI1 Monoclonal Antibody, Clone S283-7. Load: 10 ug. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.)
Mouse LGI1 Monoclonal Antibody | anti-Lgi1 antibody
LGI1 Antibody: HRP
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa LGI1 protein using Mouse Anti-LGI1 Monoclonal Antibody, Clone S283-7. Load: 10 ug. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.)
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa LGI1 protein using Mouse Anti-LGI1 Monoclonal Antibody, Clone S283-7. Load: 10 ug. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.)
Scientific Background: The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist; this LGI1 antibody will recognize only the longer form.
NCBI and Uniprot Product Information
Uniprot Description
LGI1: Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors. Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival. Defects in LGI1 are the cause of epilepsy, familial temporal lobe, type 1 (ETL1). A focal form of epilepsy characterized by recurrent seizures that arise from foci within the temporal lobe. Seizures are usually accompanied by sensory symptoms, most often auditory in nature. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region; synapse; cell junction
Molecular Function: protein binding
Biological Process: axon guidance; positive regulation of cell growth; protein homooligomerization; neurite development; positive regulation of synaptic transmission
Research Articles on Lgi1
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Product Notes
The Lgi1 lgi1 (Catalog #AAA803411) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LGI1 Antibody: HRP reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LGI1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). 1:1000 WB. Researchers should empirically determine the suitability of the Lgi1 lgi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LGI1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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