Rabbit anti-Human, Mouse GRHL3 Polyclonal Antibody | anti-GRHL3 antibody

GRHL3 Antibody

Cat. Num. AAA7050879

• Gene Names: GRHL3; SOM; VWS2; TFCP2L4
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: GRHL3; Polyclonal Antibody; GRHL3 Antibody; Grainyhead-like protein 3 homolog; Sister of mammalian grainyhead; Transcription factor CP2-like 4; anti-GRHL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 556

• Applicable Applications for anti-GRHL3 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Grainyhead-like protein 3 homolog protein (31-182AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-101968 / sc-131537 / sc-131538
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:A549 whole cell lysate,HepG2 whole cell lysate,Mouse heart tissueAll lanes: GRHL3 antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 71,69,63,58,68 kDaObserved band size: 71,69 kDa)

Immunofluorescence (IF)

(Immunofluorescent analysis of PC3 cells using MBS7050879 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-GRHL3 antibody

Transcription factor playing important roles in primary neurulation and in the differentiation of stratified epithelia of both ectodermal and endodermal origin (By similarity). Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes (PubMed: 21081122, PubMed: 25347468). xhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair (By similarity). Exhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair but is essential to form the epidermal barrier with TGM3 as critical direct target gene among others. Despite being dispensable during normal epidermal homeostasis in the adulthood, is again required for barrier repair after immune-mediated epidermal damage, regulates distinct gene batteries in embryonic epidermal differentiation and adult epidermal barrier reformation after injury. Plays unique and cooperative roles with GRHL2 in establishing distinct zones of primary neurulation. Essential for spinal closure, functions cooperatively with GRHL2 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (By similarity). Also required for proper development of the oral periderm (PubMed: 24360809). No genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity (PubMed: 21081122).

NCBI and Uniprot Product Information

• NCBI GI #: 303324555
• NCBI GeneID: 57822
• NCBI Accession #: NP_001181939.1
• NCBI GenBank Nucleotide #: NM_001195010.1
• UniProt Accession #: Q8TE85; Q5TH78; Q86Y06; Q8N407; A2A297; B2RCL1; G3XAF0
• Molecular Weight: 67,696 Da
• NCBI Official Full Name: grainyhead-like protein 3 homolog isoform 4
• NCBI Official Synonym Full Names: grainyhead like transcription factor 3
• NCBI Official Symbol: GRHL3
• NCBI Official Synonym Symbols: SOM; VWS2; TFCP2L4
• NCBI Protein Information: grainyhead-like protein 3 homolog
• UniProt Protein Name: Grainyhead-like protein 3 homolog
• Protein Family: Grainyhead-like protein
• UniProt Gene Name: GRHL3
• UniProt Entry Name: GRHL3_HUMAN

NCBI Description

This gene encodes a member of the grainyhead family of transcription factors. The encoded protein may function as a transcription factor during development, and has been shown to stimulate migration of endothelial cells. Multiple transcript variants encoding distinct isoforms have been identified for this gene.[provided by RefSeq, Aug 2010]

Uniprot Description

GRHL3: May function as a transcription factor. Belongs to the grh/CP2 family. Grainyhead subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: chromatin DNA binding; protein binding; sequence-specific DNA binding

Biological Process: epidermis development; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; regulation of actin cytoskeleton organization and biogenesis

Disease: Van Der Woude Syndrome 2