Mouse anti-Human LF / LTF / Lactoferrin Monoclonal Antibody | anti-LF antibody

Mouse Monoclonal [clone 1A1] (IgG1) to Human LF / LTF / Lactoferrin

Cat. Num. AAA245291

• Gene Names: LTF; LF; HLF2; GIG12; HEL110
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: LF / LTF / Lactoferrin; Monoclonal Antibody; Mouse Monoclonal [clone 1A1] (IgG1) to Human LF / LTF / Lactoferrin; Anti-LF / LTF / Lactoferrin Antibody (clone 1A1) IHC-plus; LTF; GIG12; Growth-inhibiting protein 12; HLF2; Lactotransferrin; Lactoferrin; LF; Neutrophil lactoferrin; Talalactoferrin; Human LF; anti-LF antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1A1
• Specificity: Recognizes human lactoferrin, a 78kD globular protein found in body secretions such as milk and saliva and is a member of the transferrin family proteins. Transferrins control the level of free iron in the body, by binding and transporting iron. Lactoferrin does not appear to have a transporting function, instead it has antimicrobial activity, by keeping the levels of iron in body fluids low and thus preventing microbes from acquiring iron. Removing free iron from body fluids and inflamed areas also serve to protect against the damaging effects of free radicals. Lactoferrin is used as a therapeutic and prophylactic treatment of various illnesses, such as cancers and intestinal illnesses. May be used to detect lactoferrin in ELISA assays.
• Purity/Purification: Affinity Purified
• Form/Format: PBS, pH 7.4, 0.09% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 710

• Applicable Applications for anti-LF antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (10 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Purified lactoferrin from human milk.
• Immunogen Type: Purified protein
• Immunogen: LF / LTF / Lactoferrin antibody was raised against purified lactoferrin from human milk.
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-LTF / Lactoferrin antibody IHC of human breast, ductal with secretion. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 54607120
• NCBI GeneID: 4057
• NCBI Accession #: NP_002334.2
• NCBI GenBank Nucleotide #: NM_002343.4
• UniProt Accession #: P02788; O00756; Q16780; Q16785; Q16786; Q16789; Q5DSM0; Q8IU92; A8K9U8; B2MV13; B7Z4X2; E7EQH5
• Molecular Weight: 73,161 Da
• NCBI Official Full Name: lactotransferrin isoform 1
• NCBI Official Synonym Full Names: lactotransferrin
• NCBI Official Symbol: LTF
• NCBI Official Synonym Symbols: LF; HLF2; GIG12; HEL110
• NCBI Protein Information: lactotransferrin; kaliocin-1; lactoferricin; lactoferroxin; talalactoferrin; neutrophil lactoferrin; growth-inhibiting protein 12; epididymis luminal protein 110
• UniProt Protein Name: Lactotransferrin
• UniProt Gene Name: LTF
• UniProt Synonym Gene Names: GIG12; LF; Lactoferrin; Lfcin-H
• UniProt Entry Name: TRFL_HUMAN

NCBI Description

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 3.4.21.-; Secreted; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule

Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; ferric iron binding; DNA binding; iron ion binding; serine-type endopeptidase activity

Biological Process: interaction with host; negative regulation of lipopolysaccharide-mediated signaling pathway; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; activation of NF-kappaB transcription factor; regulation of cytokine production; positive regulation of osteoblast differentiation; retinal homeostasis; innate immune response in mucosa; negative regulation of viral genome replication; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; iron assimilation by chelation and transport; negative regulation of ATPase activity; negative regulation of viral reproduction; negative regulation of apoptosis