Western Blot (WB)
(This antibody detected recombinant LIF protein on Western Blot.)
Mouse Leukemia inhibitory factor (LIF) Monoclonal Antibody | anti-LIF antibody
Mouse Anti-Human LIF Monoclonal Antibody
Reactivity
Human, others not tested.
Applications
ELISA, Western Blot
Purity
Protein G affinity purified
Synonyms
Leukemia inhibitory factor (LIF); Monoclonal Antibody; Mouse Anti-Human LIF Monoclonal Antibody; mAb anti-human LIF; Clone 3F4; LIF alpha subunit; anti-LIF antibody
Host
Mouse
Reactivity
Human, others not tested.
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
3F4
Specificity
This antibody is reactive to recombinant human LIF protein.
Purity/Purification
Protein G affinity purified
Form/Format
Lyophilized from a solution in 0.01M PBS, pH 7.0.
Applicable Applications for anti-LIF antibody
ELISA (EIA), Western Blot (WB)
Application Notes
The antibody can detect less than 10ng/well of recombinant LIF on the blot.
EIA: This antibody is reactive to recombinant human LIF in indirect ELISA.
EIA: This antibody is reactive to recombinant human LIF in indirect ELISA.
Product Type
Primary antibody
Target Protein
LIF
Immunogen
Recombinant LIF
Fusion Myeloma
Sp2/0-Ag14
Reconstitution
Double distilled water is recommended to adjust the final concentration to 1mg/mL.
Research Area
Cytokine and oncology
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(This antibody detected recombinant LIF protein on Western Blot.)
Western Blot (WB)
(This antibody detected recombinant LIF protein on Western Blot.)
Related Product Information for anti-LIF antibody
Description:
Mouse monoclonal antibody to human Leukemia inhibitory factor (LIF).
Background:
LIF is a cytokine with important functions in diversified biological processes including cell differentiation, inflammatory response, neuronal development and cancer progression. LIF can induce the terminal differentiation of leukemia cells, thus it inhibits the further progression of leukemia. However, it has opposite effects on many other cancers, such as breast cancer and bladder cancer etc. Several recent studies have shown that LIF might play a significant role in pancreatic ductal adenocarcinoma (PDAC) tumorigenesis development. Treatment of pancreatic cancer by blocking LIF is currently under investigation. Research also demonstrated that elevated LIF level in pancreatic tissue and circulation was correlated with the cancer progression, indicating that LIF might be useful as a biomarker.
Mouse monoclonal antibody to human Leukemia inhibitory factor (LIF).
Background:
LIF is a cytokine with important functions in diversified biological processes including cell differentiation, inflammatory response, neuronal development and cancer progression. LIF can induce the terminal differentiation of leukemia cells, thus it inhibits the further progression of leukemia. However, it has opposite effects on many other cancers, such as breast cancer and bladder cancer etc. Several recent studies have shown that LIF might play a significant role in pancreatic ductal adenocarcinoma (PDAC) tumorigenesis development. Treatment of pancreatic cancer by blocking LIF is currently under investigation. Research also demonstrated that elevated LIF level in pancreatic tissue and circulation was correlated with the cancer progression, indicating that LIF might be useful as a biomarker.
Product Categories/Family for anti-LIF antibody
NCBI Description
This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
Research Articles on LIF
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Product Notes
The LIF (Catalog #AAA592458) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human LIF Monoclonal Antibody reacts with Human, others not tested. and may cross-react with other species as described in the data sheet. AAA Biotech's Leukemia inhibitory factor (LIF) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). The antibody can detect less than 10ng/well of recombinant LIF on the blot. EIA: This antibody is reactive to recombinant human LIF in indirect ELISA. Researchers should empirically determine the suitability of the LIF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Leukemia inhibitory factor (LIF), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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