Typical Testing Data/Standard Curve (for reference only)
Human Semaphorin 4B (SEMA4B) ELISA Kit | SEMA4B elisa kit
Human Semaphorin 4B (SEMA4B) ELISA Kit
Gene Names
SEMA4B; SemC; SEMAC
Reactivity
Human
Synonyms
Semaphorin 4B (SEMA4B); Human Semaphorin 4B (SEMA4B) ELISA Kit; SEMA4B elisa kit
Reactivity
Human
Sequence Length
837
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15-3000ng/L
Sensitivity
8.12ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SEMA4B elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Semaphorin 4B (also known as SEMA4B) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SEMA4B antibody. SEMA4B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA4B Antibody is added and binds to SEMA4B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA4B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SEMA4B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SEMA4B antibody. SEMA4B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA4B Antibody is added and binds to SEMA4B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA4B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SEMA4B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
81,403 Da
NCBI Official Full Name
semaphorin-4B isoform 1
NCBI Official Synonym Full Names
semaphorin 4B
NCBI Official Symbol
SEMA4B
NCBI Official Synonym Symbols
SemC; SEMAC
NCBI Protein Information
semaphorin-4B
UniProt Protein Name
Semaphorin-4B
Protein Family
UniProt Gene Name
SEMA4B
UniProt Synonym Gene Names
KIAA1745; SEMAC
Uniprot Description
Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Research Articles on SEMA4B
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Product Notes
The Human SEMA4B sema4b (Catalog #AAA162965) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA162965 ELISA Kit recognizes Human SEMA4B. It is sometimes possible for the material contained within the vial of "Semaphorin 4B (SEMA4B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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