Mouse anti-Human LDLR Monoclonal Antibody | anti-LDLR antibody

LDLR (Low-density Lipoprotein Receptor, LDL Receptor) (PE)

Cat. Num. AAA609177

• Gene Names: LDLR; FH; FHC; LDLCQ2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified by Protein G affinity chromatography form hybridoma culture supernatant.
• Synonyms: LDLR; Monoclonal Antibody; LDLR (Low-density Lipoprotein Receptor; LDL Receptor) (PE); Anti -LDLR (Low-density Lipoprotein Receptor; anti-LDLR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 11C32
• Specificity: Recognizes human LDLR.
• Purity/Purification: Purified by Protein G affinity chromatography form hybridoma culture supernatant.
• Form/Format: Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
• Concentration: 0.025 mg/mL (varies by lot)

• Applicable Applications for anti-LDLR antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: 10ul labels 1x10e6 cells. Validated using HepG2 cells.; ; Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.; 2. After blocking, add 10ul of L2601-02G to up to 1x10e6 cells and incubate for 30 minutes at RT.; 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.; 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with PE-labeled mouse IgG1 antibody.
• Immunogen: Recombinant protein corresponding to ala22-Arg788 from the extracellular domain of human LDLR, expressed in CHO cells
• Preparation and Storage: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Flow Cytometry (FC/FACS)

(HepG2 cells (filled histogram) using MBS609177 or isotype control (open histogram). Cell surface expression of LDL Receptor was determined by flow cytometry using 488nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 565 - 605nm.)

Related Product Information for anti-LDLR antibody

LDL R is the prototype low density lipoprotein receptor. It is a transmembrane protein that plays a role in cholesterol homeostasis by mediating the uptake of low density lipoproteins. Some of the many allelic variants of LDL R cause familial hypercholesterolemia.

Product Categories/Family for anti-LDLR antibody

Antibodies; Abs to Lipoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 4504975
• NCBI GeneID: 3949
• NCBI Accession #: NP_000518.1
• NCBI GenBank Nucleotide #: NM_000527.4
• UniProt Accession #: P01130; Q53ZD9; Q59FQ1; Q9UDH7; B4DII3; B4DJZ8; B4DR00; B4DTQ3; C0JYY8; H0YLU8; H0YNT7
• Molecular Weight: 95,376 Da
• NCBI Official Full Name: low-density lipoprotein receptor isoform 1
• NCBI Official Synonym Full Names: low density lipoprotein receptor
• NCBI Official Symbol: LDLR
• NCBI Official Synonym Symbols: FH; FHC; LDLCQ2
• NCBI Protein Information: low-density lipoprotein receptor; LDL receptor; low-density lipoprotein receptor class A domain-containing protein 3
• UniProt Protein Name: Low-density lipoprotein receptor
• Protein Family: LDLR chaperone
• UniProt Gene Name: LDLR
• UniProt Synonym Gene Names: LDL receptor
• UniProt Entry Name: LDLR_HUMAN

NCBI Description

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Uniprot Description

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: Golgi apparatus; cell surface; membrane; integral to plasma membrane; lysosome; late endosome; early endosome; plasma membrane; coated pit; endosome membrane; receptor complex; external side of plasma membrane

Molecular Function: very-low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; protein binding; low-density lipoprotein binding; clathrin heavy chain binding; calcium ion binding; glycoprotein binding

Biological Process: phototransduction, visible light; lipoprotein catabolic process; cholesterol metabolic process; receptor-mediated endocytosis; cholesterol homeostasis; cholesterol transport; viral reproduction; cholesterol absorption; endocytosis; lipoprotein metabolic process; phospholipid transport; lipid metabolic process; retinoid metabolic process

Disease: Hypercholesterolemia, Familial