Immunofluorescence/Immunocytochemistry (IF/ICC)
Mouse Lamin B1 + B2 Monoclonal Antibody
Mouse Anti-Lamin B1 + B2 antibody [X223] - Nuclear Envelope Marker
Reactivity
Mouse, Rat, Cow, Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Unpurified
Synonyms
Lamin B1 + B2; Monoclonal Antibody; Mouse Anti-Lamin B1 + B2 antibody [X223] - Nuclear Envelope Marker; Lamin B1 antibody; Lamin B2 antibody; LMN antibody; LMN B2 antibody; LMN2 antibody; LMNBantibody; LMNB1 antibody; LMNB2 antibody; anti-Lamin B1 + B2 antibody
Host
Mouse
Reactivity
Mouse, Rat, Cow, Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
X223
Specificity
Reacts with COS cells in immunocytochemistry and is said to detect the karyoskeleton. Other cell/tissue types have not been tested. In mouse the antibody detects Lamin B2 only.
Purity/Purification
Unpurified
Form/Format
Tissue culture supernatant containing 0.09% sodium azide
Concentration
10-15ug/ml (varies by lot)
Applicable Applications for anti-Lamin B1 + B2 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Optimal antibody dilution should be determined by titration, however as a guideline try; ICC/IF: Use at an assay dependent dilution WB: 1/50. Predicted molecular weight: 65 - 67 kDa
Target Antigen
Lamin B1 + B2 - Nuclear Envelope Marker
Immunogen
Full length native lamin B2 protein (purified) (Xenopus laevis) (XLKE-A6 cells)
Research Areas
Apoptosis; Cell Biology; Class V Lamins; Cytoskeleton; Cytoskeleton / ECM; Intermediate Filaments; Lamins; Nuclear Envelope; Nucleus; Subcellular Markers; Tags Cell Markers
Immunofluorescence/Immunocytochemistry (IF/ICC)
Immunofluorescence/Immunocytochemistry (IF/ICC)
References
The nuclear lamins. A multigene family of proteins in evolution and differentiation. Krohne G (Exp Cell Res.,Jan 1986) PUBMED Arruda DC et al. beta-Actin-binding complementarity-determining region 2 of variable heavy chain from monoclonal antibody C7 induces apoptosis in several human tumor cells and is protective against metastatic melanoma. J Biol Chem 287:14912-22 (2012). ICC/IF ; Human PUBMED Machiels BM et al. Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults. J Pathol 182:197-204 (1997) PUBMED Ulbert S et al. The inner nuclear membrane protein Lem2 is critical for normal nuclear envelope morphology. FEBS Lett. 2006 Nov 27;580(27):6435-41. Epub 2006 Nov 3. ICC/IF, WB (Human) PMID: 17097643
Similar Products
Product Notes
The Lamin B1 + B2 (Catalog #AAA375034) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Lamin B1 + B2 antibody [X223] - Nuclear Envelope Marker reacts with Mouse, Rat, Cow, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lamin B1 + B2 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Optimal antibody dilution should be determined by titration, however as a guideline try; ICC/IF: Use at an assay dependent dilution WB: 1/50. Predicted molecular weight: 65 - 67 kDa. Researchers should empirically determine the suitability of the Lamin B1 + B2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lamin B1 + B2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
