Immunofluorescence/Immunocytochemistry (IF/ICC)
Mouse Lamin A+C Monoclonal Antibody
Mouse Anti-Lamin A + C antibody [JoL3] - Nuclear Envelope Marker
Reactivity
Mouse, Rat, Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Unpurified
Synonyms
Lamin A+C; Monoclonal Antibody; Mouse Anti-Lamin A + C antibody [JoL3] - Nuclear Envelope Marker; 70 kDa Lamin antibody; Cardiomyopathy dilated 1A (autosomal dominant) antibody; CDCD1 antibody; CDDCantibody; Charcot Marie Tooth Disease Axonal Type 2B1 antibody; CMD1A antibody; CMT2B1 antibody; EMD2antibody; FPL antibody; FPLD antibody; HGPS antibody; IDC antibody; Lamin A/C antibody; Lamin A/C Isoform 1Precursor antibody; Lamin A/C Isoform 2 antibody; Lamin A/C Isoform 3 antibody; LDP1 antibody; LFPantibody; LGMD1B antibody; Limb girdle muscular dystrophy 1B (autosomal dominant) antibody; LMN 1 antibody; LMN Aantibody; LMN C antibody; LMN1 antibody; LMNA antibody; LMNC antibody; NY REN 32 antigen antibody; PRO1antibody; Progeria 1 (Hutchinson Gilford Type) antibody; Renal carcinoma antigen NY REN 32 antibody; Renalcarcinoma antigen NYREN32 antibody; anti-Lamin A+C antibody
Host
Mouse
Reactivity
Mouse, Rat, Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
JoL3
Specificity
Reacts with human dermal fibroblasts in immunocytochemistry. Other cell/tissue types have not been tested.It recognises the common tail domain of Lamin A+C, and the epitope is amino acids 464 - 572.
Purity/Purification
Unpurified
Form/Format
Tissue culture supernatant containing 0.1% sodium azide
Applicable Applications for anti-Lamin A+C antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Optimal antibody dilution should be determined by titration, however as a guideline try; WB: 1/50. Detects a band of approximately 60 kDa. ICC/IF: 1/10
Target Antigen
Lamin A + C antibody [JoL3] - Nuclear Envelope Marker
Immunogen
Recombinant bacterially expressed full length protein
Research Areas
Cell Biology; Class V Lamins; Cytoskeleton; Cytoskeleton / ECM; Lamins; Nuclear Envelope; Nucleus; Subcellular Markers; Tags Cell Markers
Immunofluorescence/Immunocytochemistry (IF/ICC)
Immunofluorescence/Immunocytochemistry (IF/ICC)
References
Mota S et al. Proteome analysis of a human liver carcinoma cell line stably expressing hepatitis delta virus ribonucleoproteins. J Proteomics 72:616-27 (2009). WB ; HumanPUBMED Dyer JA et al. Cell cycle changes in A-type lamin associations detected in human dermal fibroblasts using monoclonal antibodies. Chromosome Res 5:383-94 (1997)PUBMED
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Product Notes
The Lamin A+C (Catalog #AAA375228) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Lamin A + C antibody [JoL3] - Nuclear Envelope Marker reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lamin A+C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Optimal antibody dilution should be determined by titration, however as a guideline try; WB: 1/50. Detects a band of approximately 60 kDa. ICC/IF: 1/10. Researchers should empirically determine the suitability of the Lamin A+C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lamin A+C, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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