Mouse Lamin A, Cleavage Site Monoclonal Antibody | anti-LMNA antibody

Lamin A, Cleavage Site (Small Subunit)

Cat. Num. AAA600805

• Gene Names: LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Purified; Purified.
• Synonyms: Lamin A; Cleavage Site; Monoclonal Antibody; Cleavage Site (Small Subunit); Anti -Lamin A; anti-LMNA antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3A5
• Specificity: Detects endogenous levels of the small fragment of lamin A when cleaved by caspase-6 at Asp230. It is a marker of caspase-6 activity. Detects the small fragment of lamin A/C only when cleaved at Asp230. Does not crossreact with full length lamin A (nor with lamin C). Species Crossreactivity: Mouse and rat.
• Purity/Purification: Purified; Purified.
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.

• Applicable Applications for anti-LMNA antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Dilution: Western Blot: 1:1000 Immunocytochemistry (ABC) 1:1000 Immunocytochemistry (IF) 1:1000
• Immunogen: Synthetic peptide corresponding to N-terminal residues adjacent to Asp230 in human lamin A.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, untreated or staurosporine-treated (1uM), and Jurkat cells, untreated or etoposide-treated (25mM), using Cleaved Lamin A (Small Subunit) (30H5) Monoclonal Antibody.)

Immunocytochemistry (ICC)

(Immunocytochemical staining of HeLa cells, untreated (right) or staurosporine-treated (left), using Cleaved Lamin A (Small Subunit) (30H5) Monoclonal Antibody.)

Related Product Information for anti-LMNA antibody

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A is cleaved by caspase-6 and serves as a marker for caspase- 6 activation. During apoptosis, the 70kD lamin A is specifically cleaved to a large (40-45kD) and a small (28kD) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

Product Categories/Family for anti-LMNA antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 27436946
• NCBI GeneID: 4000
• NCBI Accession #: NP_733821.1
• NCBI GenBank Nucleotide #: NM_170707.3
• UniProt Accession #: P02545; P02546; Q5TCJ2; Q5TCJ3; Q969I8; Q96JA2; B4DI32; D3DVB0; E7EUI9
• Molecular Weight: 74,139 Da
• NCBI Official Full Name: lamin isoform A
• NCBI Official Synonym Full Names: lamin A/C
• NCBI Official Symbol: LMNA
• NCBI Official Synonym Symbols: FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
• NCBI Protein Information: lamin; 70 kDa lamin; prelamin-A/C; lamin A/C-like 1; renal carcinoma antigen NY-REN-32
• UniProt Protein Name: Prelamin-A/C
• Protein Family: Lamin
• UniProt Gene Name: LMNA
• UniProt Synonym Gene Names: LMN1
• UniProt Entry Name: LMNA_HUMAN

NCBI Description

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]

Uniprot Description

lamin A/C: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. Cleaved by caspase-6 during apoptosis into a 40-45 kDa and a28 kDa fragment.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: nucleoplasm; nuclear lamina; nuclear membrane; perinuclear region of cytoplasm; cytoplasm; lamin filament; intermediate filament; nuclear speck; nuclear envelope; cytosol; nucleus

Molecular Function: protein binding; structural molecule activity

Biological Process: mitotic nuclear envelope reassembly; muscle development; apoptosis; unfolded protein response; ventricular cardiac muscle cell development; mitotic nuclear envelope disassembly; regulation of cell migration; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; sterol regulatory element binding protein nuclear translocation; mitotic cell cycle; establishment and/or maintenance of microtubule cytoskeleton polarity; cell structure disassembly during apoptosis

Disease: Heart-hand Syndrome, Slovenian Type; Emery-dreifuss Muscular Dystrophy 2, Autosomal Dominant; Restrictive Dermopathy, Lethal; Mandibuloacral Dysplasia With Type A Lipodystrophy; Muscular Dystrophy, Congenital, Lmna-related; Charcot-marie-tooth Disease, Axonal, Type 2b1; Emery-dreifuss Muscular Dystrophy 3, Autosomal Recessive; Lipodystrophy, Familial Partial, Type 2; Hutchinson-gilford Progeria Syndrome; Muscular Dystrophy, Limb-girdle, Type 1b; Cardiomyopathy, Dilated, 1a; Cardiomyopathy, Dilated, With Hypergonadotropic Hypogonadism