anti-Human, Mouse Lactoferrin Monoclonal Antibody | anti-LTF antibody

Mouse anti Lactoferrin

Cat. Num. AAA570219

• Gene Names: LTF; LF; HLF2; GIG12
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot
• Synonyms: Lactoferrin; Monoclonal Antibody; Mouse anti Lactoferrin; anti-LTF antibody

• Host: Mouse (Balb/c)
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 67D9
• Specificity: Monoclonal antibodies 67D2, 67D9 and 67F12 bind to the same or overlapping epitopes on an 80 Kd antigen indiCated as MAM-5. On frozen as well as on formalin-fixed lactating mammary gland sections, a very strong reaction in secretion products is observed. Occasionally, preexisting ducts react in some of the mammary tumors, indiCating an enhanced secretory activity in these parts of the mammary gland.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-LTF antibody: Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
• Application Notes: 67D2 is useful for western blot and immunohistochemistry on frozen and paraffin embedded sections. Optimal antibody dilution should be determined by titRation.
• Source Note: 67D9 is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with milkfat globule membranes.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Related Product Information for anti-LTF antibody

Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is a globular glycoprotein with a molecular mass of about 80 kDa that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. Lactoferrin is also present in secondary granules of polymorphonuclear neutrophils (PMN) and is secreted by some acinar cells. The identification and classifiCation of Human breast carcinomas is mainly based on morphology, which has its difficulties and limitations. Specific markers for breast tumors and additional markers, to correlate with the progress of the disease, are needed. Antibodies to Human milk-fat globule (HMFG) membranes are such potential markers for breast carcinomas. HMFG membranes carry differentiation antigens of the apical site of the secretory cell which may be present also on tumor cells.

Product Categories/Family for anti-LTF antibody

Tumour marker

References

J. Hilkens, F. Buus, J. Hegers, Ph. Hageman2, J. Calafati, A. Sonnenberg and M. Vanderval. Monoclonal Antibodies anti Human Milk-Fat Globule Membranes Detecting Differentiation Antigens Of The Mammary Gland And Its Tumors. Int J Cancer. 1984 34:197-206.

NCBI and Uniprot Product Information

• NCBI GI #: 2104522
• NCBI GeneID: 4057
• UniProt Accession #: P02788; O00756; Q16780; Q16785; Q16786; Q16789; Q5DSM0; Q8IU92; Q8IZH6; B2MV13; B7Z4X2; E7EQH5
• Molecular Weight: 78,182 Da
• NCBI Official Full Name: lactoferrin
• NCBI Official Synonym Full Names: lactotransferrin
• NCBI Official Symbol: LTF
• NCBI Official Synonym Symbols: LF; HLF2; GIG12
• NCBI Protein Information: lactotransferrin; talalactoferrin; neutrophil lactoferrin; growth-inhibiting protein 12
• UniProt Protein Name: Lactotransferrin
• Protein Family: Lactotransferrin
• UniProt Gene Name: LTF
• UniProt Synonym Gene Names: GIG12; LF; Lactoferrin; Lfcin-H
• UniProt Entry Name: TRFL_HUMAN

NCBI Description

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 3.4.21.-; Secreted, signal peptide; Secreted; Protease

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule

Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; DNA binding; ferric iron binding; iron ion binding; serine-type endopeptidase activity

Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; interaction with host; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; activation of NF-kappaB transcription factor; regulation of cytokine production; retinal homeostasis; positive regulation of osteoblast differentiation; negative regulation of viral genome replication; innate immune response in mucosa; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; negative regulation of viral reproduction; iron assimilation by chelation and transport; negative regulation of ATPase activity; negative regulation of apoptosis