Mouse anti-Human Ku Monoclonal Antibody | anti-XRCC6 antibody

Ku (p70/p80) (Ku (p70): 70kD subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70kD subunit, CTC box-binding factor 75kD subunit, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, Ku autoantigen, 70kD, Ku70, Kup70,

Cat. Num. AAA6123398

• Gene Names: XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
• Reactivity: Human
• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Ku; Monoclonal Antibody; Ku (p70/p80) (Ku (p70): 70kD subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70kD subunit; CTC box-binding factor 75kD subunit; CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku autoantigen; 70kD; Ku70; Kup70; ; Ku (p70): 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; 70kDa; Lupus Ku autoantigen protein p70; ML8; Thyroid autoantigen 70kD (Ku antigen); Thyroid-lupus autoantigen (TLAA); X-ray repair cross-complementing protein 6 (XRCC6) Ku (p80): 86kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 86 Kd subunit; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85kDa subunit; CTC85; DNA repair protein XRCC5; KARP1; Ku autoantigen 80kDa; Ku80; Ku86 autoantigen related protein 1; KUB2; Lupus Ku autoantigen protein p86; Nuclear factor IV (NFIV); X-ray repair cross-complementing protein 5 (XRCC5); anti-XRCC6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: KU729
• Specificity: Recognizes Ku. Species Crossreactivity: human and primate. Does not react with mouse, rat or chicken. Others not known.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.

• Applicable Applications for anti-XRCC6 antibody: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP)
• Application Notes: IF: 0.5-1.0ug/ml; IHC-F/P: 0.5-1.0ug/ml for 30 minutes at RT; IP: 0.5-1ug/500ug protein lysate; Applications are based on unconjugated antibody.
• Immunogen: Nuclear extract of human HL-60 cells. Cellular Localization: Nuclear
• Conjugate: Biotin
• Positive Control: Human cancer K562, HL-60, HEp-2 or HeLa cells
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Categories/Family for anti-XRCC6 antibody

Antibodies; DNA Replication; Repair

NCBI and Uniprot Product Information

• NCBI GI #: 4503841
• NCBI GeneID: 2547
• NCBI Accession #: NP_001460.1
• NCBI GenBank Nucleotide #: NM_001469.3
• UniProt Accession #: P12956; P13010
• Molecular Weight: 69,843 Da
• NCBI Official Full Name: X-ray repair cross-complementing protein 6
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 6
• NCBI Official Symbol: XRCC6
• NCBI Official Synonym Symbols: ML8; KU70; TLAA; CTC75; CTCBF; G22P1
• NCBI Protein Information: X-ray repair cross-complementing protein 6; 5'-dRP lyase Ku70; Ku autoantigen, 70kDa; DNA repair protein XRCC6; Ku autoantigen p70 subunit; 70 kDa subunit of Ku antigen; thyroid-lupus autoantigen p70; lupus Ku autoantigen protein p70; 5'-deoxyribose-5-pho
• UniProt Protein Name: X-ray repair cross-complementing protein 6
• Protein Family: Kurtoxin
• UniProt Gene Name: XRCC6
• UniProt Synonym Gene Names: G22P1; 5'-dRP lyase Ku70; CTC75; CTCBF; Ku70; TLAA
• UniProt Entry Name: XRCC6_HUMAN

NCBI Description

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Uniprot Description

Ku70: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Protein type: Helicase; EC 3.6.4.-; DNA-binding; DNA repair, damage; Nuclear receptor co-regulator; EC 4.2.99.-; RNA-binding

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; transcription factor complex; nuclear telomere cap complex; membrane; nucleolus; cytosol; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; double-stranded DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding

Biological Process: positive regulation of neurogenesis; V(D)J recombination; transcription, DNA-dependent; viral reproduction; positive regulation of transcription, DNA-dependent; DNA repair; double-strand break repair via nonhomologous end joining; DNA duplex unwinding; DNA ligation; double-strand break repair; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; telomere maintenance