Mouse Krueppel-like factor 4 Monoclonal Antibody | anti-KLF4 antibody

Mouse Anti Human Krueppel-like factor 4

Cat. Num. AAA140279

• Gene Names: KLF4; EZF; GKLF
• Applications: ELISA, Western Blot
• Purity: KLF4 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Krueppel-like factor 4; Monoclonal Antibody; Mouse Anti Human Krueppel-like factor 4; KLF4 Antibody; Mouse Anti Human; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor; KLF4; EZF; GKLF; anti-KLF4 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 heavy chain and kappa light chain
• Clone Number: PAT4E6AT
• Purity/Purification: KLF4 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.; Sterile Filtered clear solution.
• Sequence Length: 479

• Applicable Applications for anti-KLF4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: KLF4 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.
• Immunogen: Anti-human KLF4 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human KLF4 amino acids 1-170 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-KLF4 antibody

Introduction: KLF4 (aka the gut-enriched Kruppel-like factor-GKLF), is a zinc finger-containing transcription factor which is a member of the Kruppel-like family of transcription factors. KLF4 plays key roles during the proliferation and differentiation of epithelial cells. Particularly, it is found mainly in the gut and has been shown to be expressed during growth arrest. KLF4 is also involved in cell cycle control since expression of KLF4 inhibits DNA synthesis by blocking the G1/S phase of the cell cycle.

Product Categories/Family for anti-KLF4 antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 194248077
• NCBI GeneID: 9314
• NCBI Accession #: NP_004226.3
• NCBI GenBank Nucleotide #: NM_004235.4
• Molecular Weight: 6,489 Da
• NCBI Official Full Name: Krueppel-like factor 4
• NCBI Official Synonym Full Names: Kruppel-like factor 4 (gut)
• NCBI Official Symbol: KLF4
• NCBI Official Synonym Symbols: EZF; GKLF
• NCBI Protein Information: Krueppel-like factor 4; endothelial Kruppel-like zinc finger protein; epithelial zinc finger protein EZF; gut-enriched krueppel-like factor
• UniProt Protein Name: Krueppel-like factor 4
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF4
• UniProt Synonym Gene Names: EZF; GKLF
• UniProt Entry Name: KLF4_HUMAN

Uniprot Description

KLF4: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin

Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; phosphoinositide 3-kinase regulator activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; post-embryonic camera-type eye development; positive regulation of nitric oxide biosynthetic process; somatic stem cell maintenance; negative regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of hemoglobin biosynthetic process; regulation of phosphoinositide 3-kinase activity; positive regulation of protein metabolic process; negative regulation of cell proliferation; post-embryonic hemopoiesis; regulation of cell differentiation; epidermis morphogenesis; negative regulation of protein kinase B signaling cascade; response to retinoic acid; negative regulation of heterotypic cell-cell adhesion; stem cell maintenance; positive regulation of telomerase activity; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; mesodermal cell fate determination; negative regulation of phosphoinositide 3-kinase cascade