Rabbit anti-Human, Mouse USP45 Polyclonal Antibody | anti-USP45 antibody

USP45, ID (USP45, Ubiquitin carboxyl-terminal hydrolase 45, Deubiquitinating enzyme 45, Ubiquitin thioesterase 45, Ubiquitin-specific-processing protease 45) (PE)

Cat. Num. AAA6361981

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: USP45; Polyclonal Antibody; ID (USP45; Ubiquitin carboxyl-terminal hydrolase 45; Deubiquitinating enzyme 45; Ubiquitin thioesterase 45; Ubiquitin-specific-processing protease 45) (PE); Ubiquitin-specific-processing protease 45; anti-USP45 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-USP45 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: USP45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-338 amino acids from the Central region of human USP45.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(USP45 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the USP45 antibody detected the USP45 protein (arrow).)

Western Blot (WB)

(USP45 Antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the USP45 antibody detected the USP45 protein (arrow).)

Immunohistochemistry (IHC)

(USP45 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USP45 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-USP45 antibody

Antibodies; Ubiquitin

References

Quesada, V., et al. Biochem. Biophys. Res. Commun. 314(1):54-62(2004). Mungall, A.J., et al. Nature 425(6960):805-811(2003). Puente, X.S., et al. Nat. Rev. Genet. 4(7):544-558(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 122937412
• NCBI GeneID: 85015
• NCBI Accession #: NP_001073950.1
• NCBI GenBank Nucleotide #: NM_001080481.1
• UniProt Accession #: Q70EL2; Q5T062; Q86T44; Q86TC0; Q9BRU1; B2RXG0
• Molecular Weight: 91,733 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 45
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 45
• NCBI Official Symbol: USP45
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 45; ubiquitin thioesterase 45; deubiquitinating enzyme 45; ubiquitin thiolesterase 45; ubiquitin specific protease 45; ubiquitin-specific-processing protease 45
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 45
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP45
• UniProt Entry Name: UBP45_HUMAN

Uniprot Description

USP45: Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Ubiquitin conjugating system; Protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 6q16.2

Molecular Function: zinc ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination