Goat anti-Human FABP4 / AP2 Polyclonal Antibody | anti-FABP4 antibody

Goat Polyclonal to Human FABP4 / AP2

Cat. Num. AAA248564

• Gene Names: FABP4; aP2; ALBP; AFABP; A-FABP; HEL-S-104
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: FABP4 / AP2; Polyclonal Antibody; Goat Polyclonal to Human FABP4 / AP2; Anti-FABP4 / AP2 Antibody (Internal) IHC-plus; FABP4; A-FABP; AFABP; ALBP; AP2; Fatty acid-binding protein 4; Human FABP4; anti-FABP4 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human FABP4 / AP2
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 132

• Applicable Applications for anti-FABP4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (5 ug/ml), WB (0.03 - 0.1 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 14kD band observed in Human Adipose tissue lysates (calculated MW of 14.7kD according to NP_001433.1). Recommended concentration: 0.03-0.1 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide C-SVNGDVITIKSEST from an internal region of human FABP4 / AP2 (NP_001433.1). Percent identity by BLAST analysis: Hamster, Elephant, Horse (93%); Sheep, Goat, Turkey, Zebra finch, Chicken (86%).
• Immunogen Type: Synthetic peptide
• Immunogen: FABP4 / AP2 antibody was raised against synthetic peptide C-SVNGDVITIKSEST from an internal region of human FABP4 / AP2 (NP_001433.1). Percent identity by BLAST analysis: Hamster, Elephant, Horse (93%); Sheep, Goat, Turkey, Zebra finch, Chicken (86%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-FABP4 / AP2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(FABP4 antibody (0.03 ug/ml) staining of Human Adipose lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-FABP4 antibody

FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.

NCBI and Uniprot Product Information

• NCBI GI #: 4557579
• NCBI GeneID: 2167
• NCBI Accession #: NP_001433.1
• NCBI GenBank Nucleotide #: NM_001442.2
• UniProt Accession #: P15090; Q6IBA1
• Molecular Weight: 14,719 Da
• NCBI Official Full Name: fatty acid-binding protein, adipocyte
• NCBI Official Synonym Full Names: fatty acid binding protein 4, adipocyte
• NCBI Official Symbol: FABP4
• NCBI Official Synonym Symbols: aP2; ALBP; AFABP; A-FABP; HEL-S-104
• NCBI Protein Information: fatty acid-binding protein, adipocyte; fatty acid-binding protein 4; adipocyte lipid-binding protein; epididymis secretory protein Li 104; adipocyte-type fatty acid-binding protein
• UniProt Protein Name: Fatty acid-binding protein, adipocyte
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP4
• UniProt Synonym Gene Names: ALBP; A-FABP; AFABP
• UniProt Entry Name: FABP4_HUMAN

NCBI Description

FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: cytoplasm; lipid particle; nucleus

Molecular Function: transporter activity; fatty acid binding

Biological Process: cholesterol homeostasis; transport; white fat cell differentiation; triacylglycerol catabolic process; brown fat cell differentiation; negative regulation of protein kinase activity; cytokine production; negative regulation of transcription, DNA-dependent; positive regulation of inflammatory response