Hamster anti-Mouse KLRG1 Monoclonal Antibody | anti-KLRG1 antibody

KLRG1 (Killer Cell Lectin-like Receptor G1, MAFA, 2F1-Ag)

Cat. Num. AAA600971

• Gene Names: KLRG1; 2F1; MAFA; MAFA-L; CLEC15A; MAFA-2F1; MGC13600; MAFA-LIKE
• Reactivity: Mouse
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Purified; Purified
• Synonyms: KLRG1; Monoclonal Antibody; KLRG1 (Killer Cell Lectin-like Receptor G1; MAFA; 2F1-Ag); Anti -KLRG1 (Killer Cell Lectin-like Receptor G1; anti-KLRG1 antibody

• Host: Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 5K32
• Specificity: Reacts with mouse killer cell lectin-like receptor G1 (KLRG1, Mr 30-38kD).
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.

• Applicable Applications for anti-KLRG1 antibody: Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: Flow cytometry 1, 5, , Immunoprecipitation1, 5; Dilution: Flow Cytometry: 1ug/10e6 cells
• Immunogen: Activated NK (A-LAK) cells from B6 (H-2b) mice
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-KLRG1 antibody

Killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA or 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains.1, 2 These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1.3, 4 The 2F1 antibody stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).1, 5 Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors.1

Product Categories/Family for anti-KLRG1 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 47717096
• NCBI GeneID: 10219
• NCBI Accession #: NP_005801.3
• NCBI GenBank Nucleotide #: NM_005810.3
• UniProt Accession #: Q96E93; O43198; O75613; B7ZAM2
• Molecular Weight: 21,831 Da
• NCBI Official Full Name: killer cell lectin-like receptor subfamily G member 1
• NCBI Official Synonym Full Names: killer cell lectin-like receptor subfamily G, member 1
• NCBI Official Symbol: KLRG1
• NCBI Official Synonym Symbols: 2F1; MAFA; MAFA-L; CLEC15A; MAFA-2F1; MGC13600; MAFA-LIKE
• NCBI Protein Information: killer cell lectin-like receptor subfamily G member 1; MAFA-like receptor; OTTHUMP00000238502; OTTHUMP00000238503; ITIM-containing receptor MAFA-L; C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; mast cell function-associated antigen (ITIM-containing)
• UniProt Protein Name: Killer cell lectin-like receptor subfamily G member 1
• Protein Family: Killer cell lectin-like receptor subfamily
• UniProt Gene Name: KLRG1
• UniProt Synonym Gene Names: CLEC15A; MAFA; MAFAL
• UniProt Entry Name: KLRG1_HUMAN

NCBI Description

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. The protein encoded by this gene belongs to the killer cell lectin-like receptor (KLR) family, which is a group of transmembrane proteins preferentially expressed in NK cells. Studies in mice suggested that the expression of this gene may be regulated by MHC class I molecules. Alternatively spliced transcript variants have been reported, but their full-length natures have not yet been determined. [provided by RefSeq]

Uniprot Description

KLRG1: Plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: plasma membrane; integral to membrane

Molecular Function: receptor activity; carbohydrate binding

Biological Process: regulation of immune response; cell surface receptor linked signal transduction; cellular defense response; innate immune response; inflammatory response