Goat VDAC2 / POR Polyclonal Antibody | anti-VDAC2 antibody

Goat Polyclonal to Human VDAC2 / POR

Cat. Num. AAA241989

• Gene Names: VDAC2; POR
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: VDAC2 / POR; Polyclonal Antibody; Goat Polyclonal to Human VDAC2 / POR; Anti-VDAC2 / POR Antibody (C-Terminus) IHC-plus; VDAC2; B-36 vdac; HVDAC2; Porin-2; VDAC-2; Vdac6; Human VDAC2; POR; anti-VDAC2 antibody

• Host: Goat
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human VDAC2.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 294

• Applicable Applications for anti-VDAC2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:64000), IHC-P (2.5 - 5 ug/ml), WB (1:64000, 0.3 - 1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-GHKVGLALELEA from the C-terminus of human VDAC2 (NP_003366.2). Percent identity by BLAST analysis: Human, Gorilla (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: VDAC2 / POR antibody was raised against synthetic peptide C-GHKVGLALELEA from the C-terminus of human VDAC2 (NP_003366.2). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Rabbit, Horse, Pig, Opossum, Platypus (92%); Orangutan, Turkey, Chicken, Xenopus, Seabass, Catfish, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish, Mosquito, Water flea, Beetle (83%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-VDAC2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (0.3 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-VDAC2 antibody

Family: Ion Channel; Subfamily: VDAC porin

NCBI and Uniprot Product Information

• NCBI GI #: 42476281
• NCBI GeneID: 7417
• NCBI Accession #: NP_003366.2
• NCBI GenBank Nucleotide #: NM_003375.3
• UniProt Accession #: P45880; Q5VWK1; Q5VWK3; Q6IB40; Q7L3J5; Q9BWK8; Q9Y5I6
• Molecular Weight: 30,412 Da
• NCBI Official Full Name: voltage-dependent anion-selective channel protein 2 isoform 2
• NCBI Official Synonym Full Names: voltage-dependent anion channel 2
• NCBI Official Symbol: VDAC2
• NCBI Official Synonym Symbols: POR
• NCBI Protein Information: voltage-dependent anion-selective channel protein 2; outer mitochondrial membrane protein porin 2
• UniProt Protein Name: Voltage-dependent anion-selective channel protein 2
• Protein Family: Voltage-dependent anion-selective channel protein
• UniProt Gene Name: VDAC2
• UniProt Synonym Gene Names: VDAC-2; hVDAC2
• UniProt Entry Name: VDAC2_HUMAN

NCBI Description

This gene encodes a member of the voltage-dependent anion channel pore-forming family of proteins that are considered the main pathway for metabolite diffusion across the mitochondrial outer membrane. The encoded protein is also thought to be involved in the mitochondrial apoptotic pathway via regulation of BCL2-antagonist/killer 1 protein activity. Pseudogenes have been identified on chromosomes 1, 2, 12 and 21, and alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

VDAC2: Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation- selective. Interacts with hexokinases. Expressed in all tissues examined. Belongs to the eukaryotic mitochondrial porin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, misc.; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: pore complex; mitochondrial outer membrane; mitochondrion; mitochondrial inner membrane; nucleus

Molecular Function: protein binding; nucleotide binding; voltage-gated anion channel activity; porin activity

Biological Process: negative regulation of protein polymerization; transmembrane transport; anion transport