Mouse KINESIN HEAVY CHAIN Monoclonal Antibody | anti-KIF5B antibody

MOUSE ANTI KINESIN HEAVY CHAIN

Cat. Num. AAA213375

• Gene Names: KIF5B; KNS; KINH; KNS1; UKHC; HEL-S-61
• Reactivity: Chicken (Expected from Sequence), InVertebrates (Expected from Sequence), Mammals (Expected from Sequence)
• Applications: Immunohistochemistry, ELISA, Immunoblot, Western Blot, Immunoprecipitation, Immunohistochemistry, Western Blot
• Synonyms: KINESIN HEAVY CHAIN; Monoclonal Antibody; MOUSE ANTI KINESIN HEAVY CHAIN; anti-KIF5B antibody

• Host: Mouse
• Reactivity: Chicken (Expected from Sequence), InVertebrates (Expected from Sequence), Mammals (Expected from Sequence)
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: H2
• Specificity: This item recognises the heavy chain of kinesin toward the N-terminus. The epitope is located in the region of amino acids 420-445.Kinesins are microtubule associated motor proteins, responsible for converting nucleoside triphosphate released energy into mechanical energy. Kinesin-1 exists as a homodimer of 120-130kDa chains, known as heavy chains. Associated with this dimer when purified from brain homogenates are light chains of 60-70kDa. The light chains are involved in binding of kinesin to organelles and appear to have regulatory functions. They are not essential for motility generation.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 963

• Applicable Applications for anti-KIF5B antibody: Immunohistology Frozen, ELISA (EIA), Immunoblotting (IB), Immunoprecipitation (IP), Immunohistology Paraffin, Western Blot (WB)
• Application Notes: Western Blotting: Minimum Dilution: 1/500
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3)
• Immunogen: Bovine brain kinesin; Buffer Solution
• Target Species: Bovine
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Related Product Information for anti-KIF5B antibody

MBS213375 recognises the heavy chain of kinesin toward the N-terminus. The epitope is located in the region of amino acids 420-445. Kinesins are microtubule associated motor proteins, responsible for converting nucleoside triphosphate released energy into mechanical energy. Kinesin-1 exists as a homodimer of 120-130kDa chains, known as heavy chains. Associated with this dimer when purified from brain homogenates are light chains of 60-70kDa. The light chains are involved in binding of kinesin to organelles and appear to have regulatory functions. They are not essential for motility generation.

NCBI and Uniprot Product Information

• NCBI GI #: 34083
• NCBI GeneID: 3799
• UniProt Accession #: P33176; Q5VZ85; A0AVB2
• Molecular Weight: 109,685 Da
• NCBI Official Full Name: kinesin heavy chain
• NCBI Official Synonym Full Names: kinesin family member 5B
• NCBI Official Symbol: KIF5B
• NCBI Official Synonym Symbols: KNS; KINH; KNS1; UKHC; HEL-S-61
• NCBI Protein Information: kinesin-1 heavy chain; conventional kinesin heavy chain; epididymis secretory protein Li 61; kinesin 1 (110-120kD); ubiquitous kinesin heavy chain
• UniProt Protein Name: Kinesin-1 heavy chain
• Protein Family: Kinesin
• UniProt Gene Name: KIF5B
• UniProt Synonym Gene Names: KNS; KNS1; UKHC
• UniProt Entry Name: KINH_HUMAN

Uniprot Description

KIF5B: Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Belongs to the kinesin-like protein family. Kinesin subfamily.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 10p11.22

Cellular Component: ciliary rootlet; microtubule; kinesin complex; neuron projection; membrane; perinuclear region of cytoplasm; endocytic vesicle; cytoplasm; microtubule organizing center; vesicle

Molecular Function: protein binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: axon guidance; regulation of membrane potential; cellular protein metabolic process; positive regulation of potassium ion transport; vesicle transport along microtubule; positive regulation of synaptic transmission, GABAergic; cytoplasm organization and biogenesis; cytoskeleton-dependent intracellular transport; microtubule-based movement