Testing Data
Zika virus E/Envelope ELISA Pair Set | ZIKV-E elisa pair set
Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set
Synonyms
Zika virus E/Envelope; Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set; ZIKV-E elisa pair set
Specificity
Quantitative determination of ZIKV ZIKV Envelope
Assay Type
Solid Phase Sandwich ELISA
Detection Range
125-8000pg/mL
Sensitivity
125pg/ml
Materials Provided
1. Capture Antibody 0.5 mg/mL of mouse anti-Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.8ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 510 ng of recombinant Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E). Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.8ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 510 ng of recombinant Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E). Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for ZIKV-E elisa pair set
Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF. Domain III of the Zika envelope protein , likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
This Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set is a solid phase sandwich ELISA for quantitative determination of ZIKV ZIKV Envelope. It contains ZIKV ZIKV Envelope capture antibody, ZIKV ZIKV Envelope detector antibody (HRP) and a highly purified HEK293-expressed recombinant ZIKV ZIKV Envelope protein.
This Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set is a solid phase sandwich ELISA for quantitative determination of ZIKV ZIKV Envelope. It contains ZIKV ZIKV Envelope capture antibody, ZIKV ZIKV Envelope detector antibody (HRP) and a highly purified HEK293-expressed recombinant ZIKV ZIKV Envelope protein.
References
Marina Basarab specialty registrar. et al.Zika virus. BMJ 2016; 352:i1049 doi: 10.1136/bmj.i1049
Pinto Junior VL, et al. Zika virus: a review to clinicians, Acta Med Port 2015 Nov-Dec;28(6):760-765
L Zammarchi, et al.Zika virus infection in a traveler returning to Europe from Brazil, March 2015
Arturo Galindo-Fraga, et al. Zika virus: A new epidemic on our doorstep. Rev Inves Clin.2015, 67: 329-32
Pinto Junior VL, et al. Zika virus: a review to clinicians, Acta Med Port 2015 Nov-Dec;28(6):760-765
L Zammarchi, et al.Zika virus infection in a traveler returning to Europe from Brazil, March 2015
Arturo Galindo-Fraga, et al. Zika virus: A new epidemic on our doorstep. Rev Inves Clin.2015, 67: 329-32
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Product Notes
The ZIKV-E (Catalog #AAA8118461) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Zika virus E/Envelope, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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